Respons Biokimia dan Molekuler Padi Hasil Perlakuan Priming pada Uji Lapang Terbatas dalam Mengatasi Cekaman Salinitas
Muhammad Ammar Faris, Dr. Yekti Asih Purwestri, S.Si., M.Si.
2023 | Skripsi | BIOLOGI
The increasing salinity stress on agricultural lands has affected more than 33% of the world's irrigation areas. Rice, being one of the largest global food sources, is particularly sensitive to salinity. Salinity-stressed rice exhibits inhibited growth, leading to reduced harvest yields due to osmotic, ionic, and oxidative stresses. This issue has garnered global attention due to rising global food demand. One strategy to enhance rice tolerance against salinity stress is seed priming. This study aims to investigate the effect of seed priming on the tolerance response of rice grown under salinity stress conditions in field trials. The research was conducted at the samas paddy field, Bantul Regency, and at the Genetic Engineering Laboratory, UGM, from July to December 2022. A complete randomized design with two factors, cultivar (Inpari-35 and IR-64) and priming treatment (priming and non-priming) was employed, with four replications for each treatment variation. In this study, priming-treated seeds were sown and planted in the field. After 36 DAT, measurements were taken for SES scores, growth response, ISM, chlorophyll and carotenoid content, antioxidant enzyme activity, proline content, and the expression of OsSOS1 and OsHKT1 genes. The results indicated that priming influenced differential tolerance mechanisms between the two cultivars, as evidenced by variations in the expression of OsSOS1 and OsHKT1 genes in both roots and leaves. Both cultivars exhibited an increase in the membrane stability index, and in Inpari-35, this correlated with improved plant height, leaf number, and tiller number. Priming significantly improved the SES scores of IR-64, rendering it tolerant, and Inpari-35 highly tolerant to salinity stress.
Kata Kunci : Cekaman salinitas, Ekspresi gen, Priming benih, Stabilitas membran, Tanaman padi, Gene expression, Membrane stability, Rice plant, Salinity stress, Seed priming