PENGARUH METODE PERSIAPAN DAN STERILISASI FREEZE-DRIED HOMOLOGOUS PLATELET-RICH PLASMA TERHADAP KADAR TRANSFORMING GROWTH FACTOR-BETA

WIWIN WINDA K, drg. Kwartarini Murdiastuti, Sp.Perio(K), Ph.D ; drg. Sri Pramestri Lastianny, M.S, Sp. Perio(K)

2021 | Tesis-Spesialis | PERIODONSIA

Berdasarkan penelitian homologous Platelet-rich plasma(H-PRP) memberikan hasil lebih bagus dari autologous platelet-rich plasma(A-PRP) sehingga dilakukan pengembangan untuk terapi ini. Penelitian ini menggunakan H-PRP Palang Merah Indonesia(PMI), produk sisa yang jarang digunakan dan telah lolos screening Infeksi Menular lewat Transfusi Darah (IMLTD). Pembuatan H-PRP di PMI melalui dua metode, sedimentasi(S) dan single centrifugation(SC). Sediaan H-PRP di freeze-driying(FD) sehingga growth-factor yang dikandung tahan lama dan mudah diaplikasikan dalam rongga mulut. Sebelum digunakan, sediaan disterilkan dengan menggunakan radiasi sinar-gamma dosis 20 dan 25 KGy yang merupakn golden standart untuk bahan medis. Transforming growth factor-beta(TGF-beta), growth factor terbanyak dalam platelet dan membantu dalam proses wound healing.Tujuan penelitian: untuk mengetahui pengaruh metode persiapan dan sterilisasi Freeze-dried homologous platelet-rich plasma terhadap kadar transforming growth factor-beta. Pada penelitian digunakan 40 sampel FD-H-PRP, yang terdiri dari 10 FD-H-PRP metode persiapan (S) diradiasi 20 KGy,10 FD-H-PRP metode S diradiasi 25 KGy, 10 FD-H-PRP metode SC diradiasi 20 KGy dan 10 FD-H-PRP metode SC diradiasi 25 KGy. Selanjutnya diukur kadar TGF-beta dengan uji ELISA data dianalisis dengan uji non parametrik. Hasil penelitian: kadar TGF-beta menunjukkan peningkatan signifikan antara metode (S) dibandingkan (SC) dan terlihat peningkatan kadar TGF-beta antara dosis 25KGy dibandingkan dengan 20 KGy. Kesimpulan: dengan menurunnya relative centrifugation force (RVF) akan meningkatkan jumlah platelet dan faktor pertumbuhan yang terkandung, sehingga (S) lebih baik dari (SC) dan semakin besar paparan radiasi sinar-gamma yang diterima platelet, maka TGF-beta1 akan keluar lebih banyak untuk melindungi sel dari kerusakan DNA sehingga kadar dosis 25 KGy lebih tinggi dari 20 KGy

Research on homologous Platelet-rich plasma (H-PRP) gives better results than autologous platelet-rich plasma (A-PRP) to develop this therapy. This study used H-PRP Indonesian Red Cross (PMI), a leftover product rarely used and has passed the screening for transfusion-transmitted infection(TTI). The production of H-PRP at PMI uses two methods, sedimentation (S) and single centrifugation (SC). The H-PRP preparation is freeze-drying (FD) so that the growth factor it contains is durable and easy to apply in the oral cavity. Before use, the FD-H-PRP sterilized by gamma irradiation at doses of 20 and 25 KGy is the golden standards for medical materials. Transforming growth factor-beta (TGF-beta), the most significant growth factor in platelets and helps in the wound healing process. Aim: To determine the effect of Freeze-dried homologous platelet-rich plasma preparation and sterilization methods on levels of transforming growth factor-beta In this study, 40 samples of FD-H-PRP were used, consisting of 10 FD-H-PRP (S)was given 20KGy irradiation dose, 10 FD-H-PRPS given 25KGy irradiation, 10 FD-H- PRP SC was given 20KGy irradiation, and 10 FD-H-PRP SC was given 25KGy irradiation. Furthermore, the TGF-beta level was measured using the ELISA test. The data were analyzed using the non-parametric test. Results: TGF-beta levels showed a significant increase between methods (S) versus (SC), and an increase in TGF-beta levels between doses of 25KGy compared to 20 KGy was seen. Conclusion: decreasing the relative centrifugation force (RVF) will increase the number of platelets, and the growth factors contained, so that (S) is better than (SC) and the greater the-ray radiation exposure received by the platelets, the more TGF-beta1 will come out. to protect cells from DNA damage so that the dose level of 25 KGy is higher than 20 KGy

Kata Kunci : Sedimentation, single centrifugation, Freeze-Dried Homologous PRP, radiasi sinar-gamma, TGF-beta