DEGRADASI IN SACCO JERAMIPADI AMONIASI DENGAN ARAS UREA BERBEDA YANG MENDAPATKAN TRICHODERMA REESEI

Setyowati, Lucia Niken Laras , Dr. Ir. Subur Priyono Sasmito Budhi

1995 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini dilakukan untuk mengetahui degradasi in sacco jerami padi amoniasi dengan aras urea berbeda yang mendapatkan Trichoderma reesei. Pada penelitian ini digunakan 3 ekor sapi perah Peranakan Friesien Holstein kering yang difistulasi pada bagian rumen, diberi pakan terdiri dari Pennisetum purpureum dan kosentrat dengan imbangan bahan kering 70:30 serta kandungan protein kasar 12,56%, pemberian air minum secara ad libitum. Jerami padi amoniasi diperam selama 4 minggu. Jerami padi (I), jerami padi amoniasi 2%+T. reesei 5% (II), jerami padi amoniasi 4%+T. reesei 5% (III), jerami padi amoniasi 6%+T.reesei 5% (IV) diinkubasikan dalam rumen selama 2, 4, 8, 16, 24 dan 48 jam dan setiap waktu diadakan 6 kali ulangan. Residu pakan setelah inkubasi dianalisis kandungan BK (bahan kering), BO (bahan organik), dan PK (protein kasar). Kinetik kehilangan BK , BO dan PK dihitung dengan model eksponensial , p=a+b(l-exp~ct). Nilai a, b, c yang diperoleh untuk menghitung nilai degradasi dengan kp=0,06 dalam rumus DT=a+(b.c/c+0,06). Nilai a, b, c , dan DT dianalisis dengan analisis variansi Rancangan Acak Lengkap (RAL) pola searah , jika terdapat perbedaan dilakukan uji Duncan. Hasil penelitian menunjukkan peningkatan (P<0,01) nilai b dan DT dari BK, BO, PK. Nilai DT dari BK I, II , III , IV berturut-turut 30,21%; 37,91%; 42,89%; 40,87%. Nilai DT dari B0 berurutan yaitu 23,76%; 29 ,40%; 32,94%; 31,62% sedang nilai DT dari PK ialah 27,25%; 51,93% 56,33%; 55,24%. Rerata perbedaan masing-masing perlakuan untuk nilai DT menunjukkan peningkatan (P<0,01) dengan uji Duncan's, kecuali untuk nilai DT PK III vs IV (56,33% vs 55,24%) tidak meningkat secara nyata. Kata kunci: Trichoderma reesei, jerami padi , amoniasi , degradasi, in sacco.

This research was conducted to investigate in sacco degradation of different urea-ammoniated levels of rice straw treated with Trichoderma reesei. There were four treatments of different levels of urea (dry matter basis) namely treatment I without treatment as control, treatment II rice straw + 2% urea; treatment III rice straw + 4% urea and treatment IV rice straw + 6% urea. Ammoniation was done four weeks. All treatments excepts treatment I was mixed 5% of T. reesei. In sacco study was done using three fistulated Friesien Holstein Crossed breeds cows for 2; 4; 8; 16; 24; and 48 hours of six replication each treatment. The cows were fed Pennisetum purpureum and concentrate 70:30 DM ratio, CP content 12.56% to satisfy their maintenance nutrient requirements. Degradation kinetics was adjusted to exponential model, p=a+b(1-exp ct). The result of a, b, c parameters were used to calculate degradation theory (DT). Rumen out-flow rate (kp) of 0.06/h were used to calculate DT, in which DT=a+(b.c/c+kp). Data were then analized using one way Completely Randomized Design. The results showed that b and DT of DM (Dry Matter), OM (Organic Matter), and CP (Crude Protein) of treated straw increased PcO.Ol). DT of DM of treatment I, II, III and IV 30.21%; 37.91%; 42.89%; 40.87% in which DT of OM was 23.76%; 29.40%; 32.94%; 31.62% while DT of CP was 27.25%; 51.93%; 56.33%; 55.24% respectively. Duncan's test showed that the results of four treatments of DT was highly significant (P<0.01) except DT of CP treatment III vs IV (56.33% vs 55.24%) was not significant. Key words: Trichoderma reesei, rice straw, ammoniation, degradation, in sacco.

Kata Kunci : Trichoderma reesei, jerami padi , amoniasi , degradasi, in sacco