OPTIMASI FORMULA DAN UJI AKTIVITAS SECARA IN VITRO LOTION O/W EKSTRAK ETANOLIK RIMPANG TEMU MANGGA ( Curcuma mangga Val. & van. Zijp) SEBAGAI TABIR SURYA

MERCY ARIZONA, Drs. Abdul Karim Z, M.Si., Apt

2016 | Skripsi | S1 FARMASI

Ekstrak temu mangga (Curcuma mangga Val.) memiliki aktivitas sebagai tabir surya secara spektrofotometri. Penelitian ini bertujuan untuk mengetahui formula optimum lotion o/w, sifat dan stabilitas fisik formula optimum lotion o/w, serta aktivitas tabir surya formula optimum lotion o/w ekstrak temu mangga (Curcuma mangga Val.) secara spektrofotometri. Formula optimum diperoleh dengan metode Simplex Lattice Design (SLD). Respon yang digunakan untuk menentukan formula optimum adalah daya lekat dan viskositas. Stabilitas fisik lotion o/w pada suhu ruang meliputi uji daya sebar, daya lekat, dan viskositas, serta uji freeze thaw cycling. Aktivitas tabir surya lotion o/w ditentukan secara spektrofotometri untuk menentukan Sun Protecting Factor (SPF), % transmisi eritema, dan % transmisi pigmentasi. Data dianalisis dengan One Way ANOVA dan uji t. Hasil penelitian menunjukkan bahwa formula optimum lotion o/w pada kombinasi 5% trietanolamin (TEA)-stearat dan 5% setil alkohol. Lotion o/w ekstrak temu mangga memiliki daya sebar 63,21 ± 2,69 cm2, daya lekat 2,32 ± 0,15 detik, dan viskositas sebesar 133,5 ± 8,05 dPas. Hasil analisis statistika menunjukkan bahwa lotion o/w memiliki stabilitas yang baik. Lotion o/w mempunyai aktivitas sebagai tabir surya pada konsentrasi ekstrak dalam lotion 0,015% dengan nilai SPF sebesar 12,817 ± 0,161 dan tidak efektif dalam perlindungan terhadap eritema dan pigmentasi.

The extract of Curcuma mangga Val.is proven to have sunscreen activity, which was evaluated by spectrophotometry. This study aimed to identify and determine the optimum formulation of oil in water lotion of Curcuma mangga extract, properties and physical stability of the lotion. The activity of optimum formulation of sunscreen o/w lotion of Curcuma mangga extract (Curcuma mangga Val.) was measured by spectrophotometry. The optimum formulation was obtained by Simplex Lattice Design (SLD) method, then the physical characteristics was employed to create the optimum formulation. Physical stability of o/w lotion was measured at room and accelerated temperature. The sunscreen activity of o/w lotion was measured to determine the Sun Protecting Factor (SPF) value, percent of erythema transmission and percent of pigmentation transmission. The result showed that the optimum formulation of o/w lotion was obtained from combination of 5% triethanolamine (TEA)-stearic and 5% cetyl alcohol. Oil in water lotion of Curcuma mangga extract has a good stability, spreadability was 3.21 ± 2.69 cm2, while adhesion power and viscosity was 2.32 ± 0.15 seconds and 133.5 ± 8.05 dPas respectively. The SPF value was 12.817 ± 0.161, indicating that Curcuma mangga extract in optimum formulation of o/w lotion can be considered as a good candidate for sunscreen. However, it was ineffective in the protection of erythema and pigmentation condition.

Kata Kunci : Curcuma mangga , lotion, SLD, tabir surya/Curcuma mangga, lotion, SLD, sunscreen