PENGARUH VARIASI KONSENTRASI TETRAHIDROPENTAGAMAVUNON-0 (THPGV-0) TERHADAP SIFAT FISIK DAN AKTIVITAS ANTIOKSIDAN SEDIAAN GEL

MAS AJIE ALAMSYAH, Dr. Mimiek Murrukmihadi, SU., Apt.;Dr.Ritmaleni., S.Si.

2016 | Skripsi | S1 FARMASI

Sifat fisik sediaan gel dapat dipengaruhi oleh beberapa faktor, salah satunya yaitu bahan aktif. Tetrahidropentagamavunon-0 (THPGV-0) mempunyai aktivitas antioksidan. Aktivitas antioksidan THPGV-0 dalam sediaan gel bergantung pada konsentrasi THPGV-0. Penelitian bertujuan untuk mengetahui pengaruh variasi konsentrasi THPGV-0 terhadap sifat fisik dan aktivitas antioksidan sediaan gel. Senyawa THPGV-0 hasil sintesis, diisolasi dan diidentifikasi. Formula gel dibuat dengan variasi konsentrasi THPGV-0 (0,000%, 0,020%, 0,024% dan 0,030%) dan gel vitamin E sebagai kontrol positif. Uji sifat fisik meliputi organoleptis, homogenitas dan pH yang dipaparkan secara deskriptif, sementara viskositas, daya sebar dan daya lekat dianalisis menggunakan uji Saphiro-Wilk dan one-way ANOVA dengan taraf kepercayaan 95%. Aktivitas antioksidan diuji dengan metode penangkapan radikal bebas DPPH (Diphenylpicrylhydrazil) dan metode reduksi ion feri. Persen penangkapan radikal DPPH dan persen FRAP (Ferric Reducing Antioxidant Power) gel dibandingkan pada masing-masing seri konsentrasi yang sama. Hasil penelitian menunjukkan bahwa variasi konsentrasi THPGV-0 tidak berpengaruh terhadap organoleptis, homogenitas, pH dan tidak berpengaruh signifikan (p>0,05) terhadap viskositas (0,379), daya sebar (0,08) dan daya lekat (0,181). Konsentrasi THPGV-0 yang meningkat berpengaruh terhadap aktivitas antioksidan yang ditunjukkan dengan meningkatnya persen penangkapan radikal DPPH dan persen FRAP antar sediaan gel pada seri konsentrasi yang sama.

Gel can be affected by other additives. Increasing the concentration of a substance causing an increase in viscosity.Viscosity affects dispersive power and adhesion. Total phenol group on tetrahidropentagamavunon-0 (THPGV-0) is proportional to the antioxidant activity. The study aims to determine the effect of variations in the concentration of THPGV-0 toward the physical properties and antioxidant activity gel formulation. Crude product of THPGV-0 was isolated and identified. Gel compositions were made by variation concentration of THPGV-0 (0%, 0,02%, 0,024% and 0,03%) and vitamin E gel as a positive control. Physical properties of the gel such as organoleptic, homogenity and pH were analyzed descriptively while viscosity, dispersive power and adhesion were analyzed by Saphiro-Wilk test and one-way ANOVA test with a 95% confidence level. The antioxidant activity was tested by Diphenylpicrylhydrazil (DPPH) radical scavenging method and Ferric Reducing Antioxidant Power(FRAP) method. % DPPH-radical scavenging and % FRAP were compared at the same concentrations of gel. The results showed that variations in the concentration of THPGV-0 as active ingredients did not cause differences in the organoleptic, homogenity and pH also did not cause significant differences (p>0,05) in the viscosity(0.379), dispersive power (0,08) and adhesion (0.181). The increase in the concentration of THPGV-0 gave different results on antioxidant activity as indicated by the increasing of % DPPH-radical scavenging and % FRAP between gel at the same concentration.

Kata Kunci : Tetrahidropentagamavunon-0(THPGV-0),gel,sifat fisik,antioksidan