Ekspresi Receptor Advanced Gycation End-Product pada Kultur Sel Leydig Tikus Sprague Dawley yang Diinduksi oleh Advanced Glycation End-Product 200 mikrogram per mililiter dan Diberi Gamma Mangostin 5 mikromol

ADIJAYA DITA PRATAMA, Dr. dr. Dicky Moch. Rizal, Sp. And, AIFM, M.Kes ; dr. Rustamaji, M.Kes

2016 | Skripsi | S1 PENDIDIKAN DOKTER

Latar Belakang:Advanced glycation end-product (AGE) merupakan senyawa berbahaya hasil glikasi lanjutan protein dan lemak yang berperan dalam patofisiologi terjadinya atherosclerosis dan proses penuaan sel melalui proses inflamasi. Ekstrak kulit manggis mengandung senyawa gamma mangostin yang memiliki efek menurunkan respon inflamasi melalui inhibisi prostaglandin E2 (PGE2) dan menghambat lipopolisakarida. Tujuan Penelitian: mengamati efek Advanced glycation end-product dan Gamma mangostin sebagai agen antioksidan pada kultur sel Leydig Sprague Dawley dalam ekspresi Reseptor advanced glycation end-product (RAGE) Metode: Analisis experimental laboratory data kadar Ekspresi Reseptor AGE dengan menggunakan ELISA pada kultur sel Leydig tikus Sprague Dawley yang diinduksi Advanced glycation end-product (AGE) dan diberi gamma mangostin. Data diolah dengan menggunakan software SPSS Statistics 22.0 dengan uji statistik Kruskal-Wallis dan dilanjutkan uji post hoc Mann Whitney U. Hasil:Dari analisi uji kruskal wallis didapatkan hasil yang signifikan diantara ketiga kelompok (p<0.027). Perbedaan bermakna didapatkan antara kelompok 1 dan 2, dan kelompok 1 dan 3 berdasarkan uji post hoc man whitney. Kadar RAGE pada kelompok 2 (11,38643 ng/105 sel/24 jam)mengalami penurunan dibanding kelompok 1 (14,4704 ng/105 sel/ 24 jam). Dan kelompok 3 memiliki nilai paling rendah (8,293333 ng/105 sel/24 jam) Kesimpulan: RAGE pada kultur sel Leydig yang diinduksi Advanced glycation end-product (AGE) mengalami penurunan dibanding yang tidak diberi dan pemberian gamma mangostin menurunkan kadar ekspresi RAGE pada kultur sel Leydig yang diinduksi AGE

Background: Advanced glycation end-product (AGE)is a toxic substance product of advanced glycation protein and lipid that have role in pathophysiology atherosclerosis and aging cell by means of inflamation. Extract mangosteen consist of gamma mangostin that have effect reduce inflamation by means of inhibit prostaglandin E2 (PGE2) and inhibit lipopolisakarida Objectives: To observe effect of Advanced glycation end-product and Gamma mangostin as antioxidant agent on Leydig cell culture of Sprague Dawley rat Methods: Experimental laboratory analysis on Expression Receptor AGE level data of leydig cell culture sprague dawley induced by Advanced glycation end-product and given gamma mangostin with ELISA. Data processing by using SPSS Statistics 22.0 software by Kruskal-Wallis statistical test and post hoc Mann Whitney U test Result: Kruskal wallis test inform there is a significant different between 3 group (p<0.027). The different between group 1 and 2, and group 1 and 3 after post hoc man whitney test. Level RAGE group 2 (11,38643 ng/105 cell/ 24 hour) decrease if compare group 1(14,4704 ng/105 cell/ 24 hour). And group 3 has the lowest level (8,293333 ng/105 cell/ 24 hour). Conclusion: RAGE level of culture leydig cell induced by AGE lower than control and given gamma mangostin reduce level RAGE of culture leydig cell

Kata Kunci : Receptor Advanced Glycation End Product(RAGE), kultursel Leydig, tikus Sprague Dawley, Advanced glycation end-product (AGE), gamma mangostin