PENGARUH VARIASI pH TERHADAP PELEPASAN MONOMER DARI RESIN KOMPOSIT NANOFILLER

PRISCA DHYANING DENTIANA, Dr. drg. Siti Sunarintyas, M.Kes. ; Prof. Dr. Drg. Widowati Siswomihardjo, M.S.

2016 | Skripsi | S1 PENDIDIKAN DOKTER GIGI

INTISARI Resin komposit nanofiller merupakan salah satu jenis resin komposit yang digunakan sebagai bahan restorasi. Resin komposit nanofiller memiliki partikel filler silica berukuran 1-100 nm sehingga memiliki estetik dan tingkat kehalusan yang tinggi. Monomer dari resin komposit seperti bis-GMA, UDMA dan TEGDMA dapat terlepas apabila menyentuh cairan rongga mulut. Konsentrasi pelepasan monomer dapat mempengaruhi biokompatibilitas resin komposit. Tujuan penelitian adalah untuk mengetahui pengaruh variasi pH terhadap pelepasan monomer dari resin komposit nanofiller. Sampel yang digunakan adalah resin komposit nanofiller Filtek Z250XT (3M ESPE, Seefeld, Germany). Dua belas sampel berbentuk balok (6 x 4 x 2 mm) dibagi kedalam tiga kelompok kemudian direndam dengan larutan plus minus pH 3,5 (asam sitrat), plus minus pH 7 (akuades), dan plus minus pH 8 (sorensens fosfat) selama 7 hari. Setelah 7 hari perendaman, larutan perendam dianalisis menggunakan HPLC untuk mengetahui konsentrasi pelepasan monomer. Monomer dianalisis menggunakan mobile phase berupa asetonitril dan air dengan perbandingan 7:3, flow rate 1 ml/menit pada suhu ruangan. Kolom yang digunakan adalah C18 5 mikrometer, panjang 125 mm, berdiameter 4 mm. Panjang gelombang yang digunakan 220 nm. Data diuji secara statistik menggunakan ONE-WAY ANOVA. Rerata konsentrasi pelepasan monomer (mikrogram/ml) pada 3 kelompok pH ( plus minus pH 3,5; plus minus pH 7; dan plus minus pH 8) adalah 3,65 plus minus 0,57; 1,50 plus minus 0,67; dan 4,07 plus minus 0,91. Hasil uji ONE-WAY ANOVA menunjukkan terdapat perbedaan rerata konsentrasi pelepasan monomer antara tiga kelompok pH (probabilitas<0,05). Kesimpulan dari penelitian ini adalah perendaman resin komposit nanofiller dalam larutan asam dan basa meningkatkan pelepasan monomer bis-GMA dari resin komposit nanofiller.

ABSTRACT Nanofilled composite resin is a kind of composite resin that is used for restorative material. Nanofilled composite resin has silica filler particle size of 1-100 nm, providing high level of aesthetic and refinement properties. Monomer from composite resin such as bis-GMA, UDMA dan TEGDMA can be released when in contact with oral fluid. Concentration of monomer release may affect the biocompatibility of composite resin. The objective of this study was to determine the effect of pH variation on monomer release of nanofilled composite resin. The samples used were nanofilled composite resin Filtek Z250XT (3M ESPE, Seefeld, Germany). Twelve samples (6 x 4 x 2 mm) were divided into three groups and then soaked into solution of plus minus pH 3.5 (citric acid), plus minus pH 7 (distilled water), and plus minus pH 8 (sorensens phosphate) for 7 days. After 7 days of immersion, each of the solutions was analyzed using HPLC to determine the concentration of monomer release. Monomer was analyzed using a mobile phase of acetonitrile and water with ratio of 7:3, flow rate 1 ml/min at room temperature. The column used was C18 5 micrometer, 125 mm in length and 4 mm in diameter. The wavelength used was 220 nm. Data were statistically analyzed using ONE-WAY ANOVA. The means concentration of monomer release (microgram/ml) on the 3 (three) pH groups ( plus minus pH 3.5; plus minus pH 7; plus minus pH 8) were 2.97 plus minus 1.13; 1.50 plus minus 0.67; and 4.07 plus minus 0.91. Results of ONE-WAY ANOVA showed that there were differences between means concentration of monomer release from the three groups (probability<0.05). The conclusion of this study was the immersion of nanofilled composite resin in an acid solution and alkaline solution increased the bis-GMAmonomer release from nanofilled composite resin.

Kata Kunci : resin komposit nanofiller, pH, asam sitrat, sorensens fosfat, pelepasan monomer / nanofilled composite resin, pH, citric acid, sorensens phosphate, monomer release