EKSPRESI CD10 DAN AKT PADA AMELOBLASTOMA TIPE PLEKSIFORM, FOLIKULER DAN AKANTOMATOSA

YUSTITIE MARTYA HERMAWATIE, drg. Prihartiningsih, SU., Sp.BM(K);drg. Rahardjo, SU., SpBM

2016 | Tesis-Spesialis | SP ILMU BEDAH MULUT

Ameloblastoma merupakan tumor epitel odontogenik rahang, jinak, invasif lokal, agresif, dengan tingkat kekambuhan tinggi. Varian histopatologis ameloblastoma yang sering dijumpai adalah tipe pleksiform, folikular, dan akantomatosa. Penelitian bertujuan mengamati perbedaan dan hubungan ekspresi CD10 dan AKT ketiga tipe ameloblastoma dan menilai agresivitasnya. Sampel penelitian adalah blok parafin penderita ameloblastoma tipe pleksiform, folikuler dan akantomatosa di laboratorium Patologi Anatomi RS. Sardjito, Yogyakarta, Januari 2010 sampai Agustus 2015, didapatkan 23 sampel memenuhi kriteria inklusi kemudian diwarnai HE, imunohistokimia CD10 dan AKT. Penilaian imunoskor untuk melihat perbedaan dan hubungan imunoekspresi CD10 dan AKT. Uji Kruskal-Wallis menunjukkan perbedaan imunoekspresi CD10 dan AKT pada ameloblastoma tipe pleksiform, folikuler dan akantomatosa. Uji Mann Whitney menunjukkan hasil imunoskor CD10 pleksiform lebih tinggi dari folikuler, antara folikuler dan akantomatosa serta antara pleksiform dan akantomatosa perbedaan tidak bermakna. Imunoskor AKT folikuler lebih tinggi dari pleksiform dan akantomatosa lebih tinggi dari pleksiform, sedangkan antara folikuler dan akantomatosa tidak menunjukkan perbedaan bermakna. Uji Rank-Spearman menunjukkan terdapat hubungan antara CD10 dan AKT tiap-tiap tipe ameloblastoma. Koefisien korelasi folikuler lebih kuat daripada pleksiform. Akantomatosa menunjukkan hubungan CD10 dan AKT tetapi tidak bermakna. Berdasarkan analisis statistik ketiga tipe ameloblastoma menunjukkan sifat agresif, namun tipe folikuler menunjukkan sifat agresif lebih tinggi dibandingkan pleksiform dan akantomatosa.

Ameloblastoma is a common odontogenic tumor located in jaws, benign but agresif and locally invasive which has high recurrent rate.. There are many histolopathological variant of ameloblastoma. Plexiform, follicular and acanthomatous are the most common type of ameloblastoma according to histopathologycal variant. The aim of study is to make an observation of the differetiation and correlation about CD10 and AKT expression in three different histopathological type of ameloblastoma and to evaluate aggresiveness of them. All the subject were derrived from laboratorium of pathology Dr. Sardjito Hospital, in form of parafin block of ameloblastoma of Prof. Dr. Sardjito Hospital, Yogyakarta, January 2010 until August 2015. There are 23 inclusion samples with three main histopathological be the subjects, each subjects stained by HE, CD10 and AKT, and the immunoscore taken to compare the immunoexpression and correlation between CD10 and AKT in each group. The result of Kruskal-Wallis test reveals that there is a significant difference of CD10 and AKT immunoscore between group of plexiform, follicular and acanthomatous. Mann Whitney test reveals that plexiform CD10 immunoscore higher than follicular, but there is no significant difference CD10 immunoscore between follicular and acanthomatous, and between plexiform and acanthomatous. Mann Whitney test reveals that follicular AKT immunoscore higher than plexiform, and acanthomatous AKT immunoscore higher than plexiform but there is no significant difference AKT immunoscore between follicular and acanthomatous. Rank-Spearman test reveals that there is correlation between CD10 and AKT in each ameloblastoma type. Follicular has stronger coefficient correlation than plexiform, otherwise in acanthomatous type there were a correlation of CD10 and AKT but not significant. Based on statistical analysis all the type of ameloblastoma show aggresivity, but follicullar type more aggresive character than plexiform and acanthomatous.

Kata Kunci : CD10, AKT, ameloblastoma, tipe pleksiform, tipe folikular, tipe akantomatosa,CD10, AKT, ameloblastoma, plexiform, folicullar, acanthomatous.