Efek Pemberuan Vitamin D Terhadap Ekspansi Myofibroblast dan Fibroblast pada Model Unilateral Ureteral Obstruction pada Mencit

PANDHU AL AFGHANI H, Dr. dr. Dwi Cahyani Ratna Sari, M. Kes, PA(K);dr. Nur Arfian, Ph.D

2015 | Skripsi | S1 PENDIDIKAN DOKTER

Latar Belakang: Prevalensi penyakit ginjal kronis (PJK) di dunia terus bertambah dengan peningkatan sebanyak 7% setiap tahunnya. Penderita PJK memiliki risiko untuk mengalami masalah kardiovaskular dan mortalitas yang tinggi. Selama satu dekade terakhir banyak penelitian yang telah membuktikan bahwa vitamin D dan analognya memiliki peran renoprotektif dan menurunkan efek cedera pada ginjal di beberapa model penyakit ginjal. Tujuan: Mengetahui efek pemberian vitamin D terhadap ekspansi myofibroblast dan ekspansi fibroblast pada model unilateral ureteral obstruction (UUO) Metode: UUO pada mencit menginduksi terjadinya fibrosis interstitial ginjal. Ekspansi myofibroblast dinilai dengan persentese fraksi area pada tampakan histologis dengan pewarnaan immunohistokimia, sedangkan ekspansi fibroblast dinilai melalui jumlah sel di preparat histologis dengan pewarnaan immunisitokimia Hasil: Pemberian vitamin D menurunkan ekspansi myofibroblast dan ekspansi fibroblast dengan signifikan pada kelompok UUO VD dibandingkan kelompok UUO 14 (p<0.01) serta terdapat korelasi yang kuat antara ekspansi myofibroblast dan ekspansi fibroblast (r=0,870;p<0,01) Kesimpulan: Vitamin D dapat menurunkan ekspansi myofibriblast dan ekspansi fibroblast secara signifikan pada model unilateral ureteral obstruction. Terdapat korelasi antara ekspansi myofibroblast dan ekspansi fibroblast Kata kunci: vitamin D, myofibr0blast, fibroblast, fibrosis interstitial, unilateral ureteral obstruction

Backgroud: The prevalence of Chronic Kidney Disease (CKD) is increasing 7% every year worldwide. Patients with CKD have high risk of cardiovascular problems and mortality. In the last decade, many researches had shown the renoprotective role and the ability of vitamin D and its analogs to attenuate renal injury in several models of kidney diseases. Purpose: To observe the effect of vitamin D supplementation towards expansion of myofibroblasts and fibroblasts in unilateral ureteral obstruction models Method: UUO performed on mice induced kidney interstitial fibrosis. Expansion of myofibroblasts was measured in percentage of fraction area in specimens with immunohistochemistry staining. Meanwhile expansion of fibroblast was measured in total amount of cell shown in specimen with immunohistochemistry staining Result: Vitamin D significantly reduced the expansion of myofibroblast and fibroblast in UUO VD group compared to UUO 14 group (p<0.01) and strong correlation between expansion of myofibroblast and fibroblast did exist (r=0,870;p<0,01) Conclusion: Vitamin D was able to reduced the expansion of myofibroblast and fibroblast in unilatertal ureteral obstruction models and there was a correlation between expansion of myofibroblast and fibroblast Keywords: vitamin D, myofibroblast, fibroblast, kidney interstitial fibrosis, unilateral ureteral obstruction

Kata Kunci : vitamin D, myofibroblast, fibroblast, kidney interstitial fibrosis, unilateral ureteral obstruction