AKTIVITAS ANTIPLASMODIUM SENYAWA (E)-1-(4-AMINOFENIL)-3-(2, 5-DIMETOKSIFENIL)PROP-2-EN-1-ONE TERHADAP Plasmodium falciparum (FCR-3) SECARA In Vitro

RENALDO FAISAL HUDA, Dr. dr. Eti Nurwening Sholikhah., M.Kes; Prof. Dr. Mustofa, M.Kes., Apt.

2016 | Skripsi | S1 PENDIDIKAN DOKTER

Kalkon merupakan prekursor flavonoid dan isoflavonoid yang diketahui memiliki aktivitas antiplasmodium. Licochalcone A menginhibisi pertumbuhan kedua strain P. falciparum sensitif klorokuin (3D7) dan resistan klorokuin (Dd2) secara in vitro. Berdasarkan doking molekular secara in silico, telah disintesis beberapa senyawa turunan kalkon. Salah satu senyawa hasil sintesis tersebut adalah (E)-1-(4-aminofenil)-3-(2,5-dimetoksifenil)prop-2-en-1-one) yang belum diketahui aktivitas antiplasmodiumnya. Tujuan penelitian untuk mengetahui Inhibitory Consentratrion 50% (IC50)dari senyawa(E)-1-(4- aminofenil)-3- (2,5- dimetoksifenil) prop-2-en-1-one. Uji aktivitas antiplasmodium dilakukan pada kultur P. falciparum strain FCR-3 dengan metode mikroskopis. Kultur Plasmodium dibagi menjadi grup perlakuan, kontrol positif dan kontrol negatif. Berbagai seri kadar senyawa uji ditambahkan ke grup perlakuan dengan replikasi tiga kali. Nilai parasitemia dihitung dari sediaan apus kultur yang diwarnai dengan Giemsa. Hasilnya digunakan untuk menghitung persentase penghambatan pertumbuhan Plasmodium. Hasil penelitian menunjukkan bahwa, senyawa (E)-1-(4-aminofenil)-3-(2,5-dimetoksifenil)prop-2-en-1-one) mempunyai aktivitas antiplasmodium pada P. falciparum strain FCR-3 secara in vitro dengan IC50 4,66 ± 4,38 μM.

Chalcones are precursor of flavonoids and isoflavonoids that known to have antiplasmodial activity. Licochalcone A inhibited the in vitro growth of both chloroquine-sensitive (3D7) and chloroquine-resistant (DD2) strains of P. falciparum. Based on in silico molecular docking, we synthesized several chalcone derivate compounds. One of the compounds synthesized are (E) -1- (4-aminophenyl) -3- (2,5-dimethoxyiphenyl) prop-2-en-1-one) which its antiplasmodial activity not yet researched. The aim of research to find out Inhibitory Concentration 50% (IC50) of the (E) -1- (4-aminophenyl) -3- (2,5-dimethoxyiphenyl) prop-2-en-1-one) compund. Antiplasmodial activity assay are performed with FCR-3 strains of P .falciparum culture using microscopic method. Cultures were divided into trial group, positive control and negative control group. Various concentration of test compound is added to the trial group and replicated three times. Parasitemia values arecalculated from culture smears stained with Giemsa. The results are used to calculate the inhibition percentage of the Plasmodium growth. The results showed the compound (E) -1- (4-aminophenyl) -3- (2,5-dimethoxyiphenyl) prop-2-en-1-one) has in vitro antiplasmodial activity against FCR-3 strain of P. falciparum with IC50 4.66 ± 4.38 μM.

Kata Kunci : Kalkon, Antiplasmodium, Plasmodium falciparum