KARAKTERISTIK MIKROBIA Alcaligenes sp. LS2T DAN KEMAMPUANNYA DALAM MEREDUKSI SENYAWA AMONIA

RICKY GUTAMA, Nanung Agus Fitriyanto, S.Pt., M.Sc., Ph.D

2015 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui karakteristik isolat Alcaligenes sp. LS2T, dan kemampuan isolat tersebut dalam mereduksi senyawa amonia melalui proses nitrifikasi. Sumber amonia yang digunakan berasal dari bahan kimia berupa (NH4)2SO4 dan bahan biologi berupa urin sapi. Penambahan senyawa (NH4)2SO4 atau urin dilakukan pada level kosentrasi 0%, 1%, 3%, 5%, 7%, 10%, 15%,. Pengujian isolat Alcaligenes sp. LS2T yang dilakukan meliputi pengujian optical density, pengujian Nessler, pengujian diameter koloni, pengecatan Gram, pengujian katalase, dan pengujian Scanning Electron Microscope (SEM). Hasil yang di pada penambahan (NH4)2SO4 5%, 7%, 10%, dan 15% serta tumbuh baikperoleh dianalisis secara deskriptif. Karakterisasi menunjukkan bahwa isolat Alcaligenes sp. LS2T mampu tumbuh dalam medium cair dengan baik pada penambahan (NH4)2SO4 3%, dan terhambat pada penambahan urin sampai 15%. Pertumbuhan pada medium padat isolat hanya mampu tumbuh pada penambahan (NH4)2SO4 1%. Alcaligenes sp. LS2T memiliki bentuk sel batang, Gram negatif, katalase positif, berwarna putih, serta mampu mereduksi amonia pada medium dengan jumlah amonia yang sedikit pada medium pertumbuhan. Kesimpulan dari penelitian adalah penambahan amonia dapat berpengaruh pada pertumbuhan Alcaligenes sp. LS2T, yaitu dapat menghambat pertumbuhannya dengan penambahan (NH4)2SO4, sedangkan penambahan urin Alcaligenes sp. LS2T dapat tumbuh semakin baik.

This study aims to determine the characteristics of isolates Alcaligenes sp. LS2T, and the ability of this strain to reduce ammonia through the process of nitrification. Ammonia source used in this study was chemical by synthenized form of (NH4)2SO4 and cow urine as organic from of nitrogen. The addition of (NH4)2SO4 or urine was at concentration level 0%, 1%, 3%, 5%, 7%, 10%, and 15%. Testing isolates Alcaligenes sp. LS2T undertaken include optical density, testing Nessler, colony diameter, Gram stain, catalase test, and Scanning Electron Microscope (SEM) examination. The result obtained were analyzed descriptively. Characterization showed that Alcaligenes sp. LS2T able to grow in a liquid medium with the addition of 3% (NH4)2SO4, and inhibited by the addition of 5%, 7%, 10%, and 15% (NH4)2SO4. strain LS2T grew well in the addition of urine to 15%. Growing on the solid medium, strain LS2T only able to grow on the addition of 1% (NH4)2SO4. Alcaligenes sp. LS2T have bacill cell shape belongs to, Gram negative bacteria, having catalase positive, white color, and it was able to reduce ammonia in the medium with a slight amount of ammonia in the growth medium. The conclusion of the study is the addition of ammonia can affect the growth of Alcaligenes sp. LS2T, which can inhibit the growth with the addition of (NH4) 2SO4, while the addition of urine Alcaligenes sp. LS2T can grow better.

Kata Kunci : Alcaligenes sp. LS2T, Reduksi Amonia, (NH4)2SO4, Karakterisasi