FORMULA OPTIMUM GEL MINYAK ATSIRI SEREH DENGAN BASIS HPMC (Hydroxypropyl Methylcellulose) DAN KARBOPOL
SURYANI TAMBUNAN, Dr. T.N. Saifullah S., M.Si., Apt.
2015 | Skripsi | S1 FARMASIMinyak atsiri sereh ( Cymbopogon citratus) terbukti memiliki khasiat sebagai antibakteri terhadap bakteri patogen, seperti Staphylococcus aureus. Minyak atsiri dari sereh diformulasikan dalam bentuk sediaan gel dengan kombinasi basis HPMC dan karbopol. Penggunaan kombinasi ini diketahui akan menghasilkan gel dengan sifat fisik yang lebih baik daripada penggunaan tunggal. Penelitian ini bertujuan untuk meneliti pengaruh variasi HPMC dan karbopol terhadaap sifat fisik gel, konsentrasi HPMC dan karbopol untuk menghasilkan formula optimum, dan stabilitas fisik gel minyak atsiri sereh selama penyimpanan. Sediaan gel dibuat dengan kadar minyak atsiri sereh 6% dengan basis HPMC dan karbopol. Masing-masing formula dibuat dan dilakukan uji sifat fisik yang meliputi organoleptis, homogenitas, pH, daya sebar dan daya lekat. Komposisi HPMC dan karbopol ditentukan melalui proses skrining dan optimasi menggunakan metode Simplex Lattice Design dengan perangkat lunak Design Expert 7.1.5. Hasil percobaan dan prediksi SLD diverifikasi dengan uji one sample t-test dengan taraf kepercayaan 95%. Formula optimum gel minyak atsiri sereh terdiri dari 4,00% HPMC dan 1,00% karbopol. Hasil uji sifat fisik gel minyak atsiri sereh diperoleh gel yang homogen dengan nilai pH 6,00 �± 0,00, viskositas 280,00 �± 26,46 dPa.S, daya sebar 9,36 �± 0,47 cm2, dan daya lekat 2,36 �± 0,10 detik. Stabilitas fisik diuji dengan metode cycling test selama 3 siklus dan dianalisis secara statistik menggunakan one-way ANOVA taraf kepercayaan 95% yang menggambarkan stabilitas gel minyak atsiri sereh. Gel minyak atsiri sereh stabil selama 3 siklus pengujian meliputi organoleptis, homogenitas, sineresis, pH, daya lekat, dan viskositas kecuali daya sebar gel yang tidak stabil.
Lemongrass (Cymbopogon citratus) essential oil is proven to have efficacy as an antibacterial against pathogenic bacteria, such as Staphylococcus aureus. Essential oils of citronella is formulated into gel dosage forms with a base combination of HPMC and carbopol. The use of this combination is known to produce a gel with physical properties better than a single use. This research aimed to investigate the effect of variations of HPMC and carbopol to the physical properties of the gel, the concentration of HPMC and carbopol to produce the optimum formula, and the physical stability of essential oil gel of citronella during storage. Gel was made by lemongrass essential oil at a concentration of 6% with base of HPMC and carbopol. Each formula were made and tested the physical properties that include organoleptic, homogenity, pH, spreadability and adhesiveness. HPMC and carbopol composition were determined through the process of screening and optimization Simplex Lattice Design methods by Design Expert 7.1.5 software. Experimental results and predictions of SLD were verified by testing one sample t-test with a 95% confidence level. The optimum formula gel of lemongrass essential oil consisting of 4.00% HPMC and 1.00% carbopol. The results test of physical properties of lemongrass essential oil gel obtained homogeneous gel with a pH value of 6.00 �± 0.00, viscosity of 280.00 �± 26.46 dPa.S, spreadability of 9.36 �± 0.47 cm2, and adhesiveness of 2.36 �± 0.10 seconds. Physical stability was tested by cycling test method for 3 cycles and statistically analyzed using one-way ANOVA level of 95% which illustrates the stability of lemongrass essential oil gel. Lemongrass essential oil gel was stable for 3 cycles of testing include organoleptic, homogeneity, syneresis, pH, adhesion, and the viscosity of the gel except spreadability was not stable.
Kata Kunci : Gel, sereh, HPMC, karbopol
