PENGARUH PENAMBAHAN LEVEL KUNING TELUR PADA BAHAN PENGENCER NaCl FISIOLOGIS TERHADAP VIABILITAS DAN MOTILITAS SPERMATOZOA ITIK TURI YANG DISIMPAN PADA SUHU 5 DERAJAT C

FEBY NILASARI, Prof. Ir. Ismaya, M.Sc., Ph.D.;Dr. Ir. Sigit Bintara, M.Si

2015 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan bahan pengencer terhadap kualitas spermatozoa itik Turi. Bahan pengencer yang digunakan NaCl fisiologis dengan penambahan kuning telur ayam kampung 8%, 10%, dan 12%. Parameter yang diamati dalam penelitian ini adalah kualitas spermatozoa itik, seperti viabilitas (%) dan motilitas (%). Data dianalisis dengan Analysis of Variance (ANOVA) rancangan acak lengkap pola faktorial 3x4. Hasil penelitian menunjukkan bahwa kualitas dan kuantitas itik Turi segar adalah volume sperma 0,48±0,08 ml, berwarna putih susu, cenderung kental, pH 7,26±0,27, konsentrasi 2,47±0,32 milyar/ml, motilitas 7,26±10,16 %, viabilitas 84,60±2,00%, dan abnormalitas 42,82±8,13%. Hasil analisis statistik menunjukkan bahwa level kuning telur 8, 10, dan 12% berpengaruh nyata (p≤0,05) terhadap motilitas spermatozoa yaitu 67,80±12,31%, 67,85±12,38%, dan 60,60±13,04%, lama penyimpanan 0, 1, 2, dan 3 jam berpengaruh nyata (P≤0,05) yaitu 79,33±6,22%, 69,20±8,54%, 61,33±8,11%, dan 51,80±8,22%, tetapi tidak berpengaruh nyata terhadap viabilitas spermatozoa berupa level kuning telur 8, 10, dan 12% yaitu 63,65±10,5%, 63,30±9,25%, dan 63,80±8,58%, lama penyimpanan 0,1, 2, dan 3 jam sebesar 65,60±9,45%, 66,06±11,67%, 64,40±8,27% dan 64,93±9,77%. Interaksi antara level kuning telur ayam kampung dan lama penyimpanan menunjukkan hasil yang tidak berbeda nyata terhadap viabilitas dan motilitas spermatozoa. Dari penelitian ini dapat disimpulkan level kuning telur 10% mempunyai motilitas paling tinggi yaitu 55,40±11,10% dan rata-rata untuk viabilitas tertinggi didapat pada level kuning telur 8% yaitu sebesar 70,20±11,30% sampai penyimpanan 3 jam.

The aim of this reseach was to determine the effect of diluent on the sperm quality of Turi drakes. The diluent was used physiological NaCl with the addition of 8%, 10%, and 12% of chicken egg yolk. The parameters in this study was observed the spermatozoa quality of Turi drakes, such as viability (%) and motility (%). Data were Analyzed of Variance by (ANOVA) completely randomized factorial design 3x4. The results showed that the quality and quantity of fresh sperm on Turi drakes were in normal and good condition the volume of sperm 0,48±0,08 ml, white milk, condensed, pH 7,26±0,27, concentration 2,47±0,32 milyar/ml, motility 7,26±10,16%, viability 84,60±2,00%, dan abnormality 42,82±8,13%.The statistics analyzed showed that yolk level8, 10, and 12 % had significant difference (P≤0,05) on spermatozoa motility 67,80±12,31%, 67,85±12,38%, and 60,60±13,04%, for stored 0, 1, 2, and 3 hours respectively, and had significant (P≤0,05) 79,33±6,22%, 69,20±8,54%, 61,33±8,11%, and 51,80±8,22%,not significant that level of yolk 8, 10, and 12% on spermatozoa viability 63,65±10,5%, 63,30±9,25%, and 63,80±8,58%,for stored 0, 1, 2, and 3 hours respectively, and had no significant 65,60±9,45%, 66,06±11,67%, 64,40±8,27% and 64,93±9,77%. The had no significant differences interaction between the level of yolk and storage time. It can be concluded, the level of 10% egg yolk showed the highest spermatozoa motility is 55.40 ± 11.10 % and the average for the highest spermatozoa viability was obtained on the 8% egg yolk in the ammount of 70.20 ± 11.30 % to 3 storage.

Kata Kunci : Itik Turi, sperma, kuning telur ayam kampung, dan NaCl fisiologis / Turi drakes, sperm, chicken egg yolk, and physiological NaCl