PEMERIKSAAN LARVA LALAT PADA BANGKAI TIKUS DENGAN TRAUMA LUKA TERBUKA KEPENTINGANNYA UNTUK PERKIRAAN POST MORTEM INTERVAL

ANNISA RAHMAWATI, dr.Yudha Nurhantari,Sp.F,Ph.D ; Dr.drh.Sitti Rahmah Umniyati,S.U.

2015 | Skripsi | PENDIDIKAN DOKTER

Latar Belakang : Pada keadaan mayat dengan luka terbuka, proses pembusukan lebih mudah terjadi. Salah satu alternatif yang dapat digunakan pada pemeriksaan mayat yang telah membusuk yaitu memeriksa organisme yang berkembang biak pada mayat. Larva lalat dapat digunakan memperkirakan waktu kematian. Tujuan : Melihat tahapan pembusukan, membandingkan genus, urutan kedatangan, pertumbuhan larva lalat pada bangkai tikus kontrol dan trauma luka terbuka. Metode : Penelitian ini menggunakan desain eksperimental kohort dengan memberikan perlakuan trauma luka terbuka pada tikus. Hasil : Pada bangkai tikus kontrol, tahap initial decay(24 jam), tahap putrefaction (48-72 jam), tahap active decay (72-96 jam), tahap post decay (120 jam), tahap skeletonization (144 jam). Pada bangkai tikus dengan trauma luka terbuka, tahap initial decay (24 jam), tahap putrefaction (48 jam), tahap active decay (48-72 jam), tahap post decay (72-96 jam), tahap skeletonization (120 jam). Genus larva lalat pada bangkai tikus kontrol dan trauma luka terbuka, yaitu Calliphora sp., Chrysomya sp., dan Sarcophaga sp. Urutan kedatangan lalat pada bangkai tikus kontrol dan trauma luka terbuka adalah Calliphora sp., Chrysomya sp., Sarcophaga sp. Terdapat perbedaan bermakna secara statistik rerata panjang dan berat larva Chrysomya sp. hari ketiga, berat larva Calliphora sp. Kesimpulan: Tahap active decay, post-decay, skletonization pada bangkai tikus dengan trauma luka terbuka lebih cepat 24 jam dibandingkan dengan bangkai tikus kontrol. Terdapat perbedaan panjang dan, berat larva Chrysomya sp., berat larva Calliphora sp. hari ketiga antara perlakuan trauma luka terbuka dan kontrol.

Background : In the situation of the corpse with open wounds, stage of decay occur more easily. One of the alternative that can be used in the examination on the corpse had decayed, examine the organisms that breed on the corpse. Fly larvae can be used to estimate the time of death (postmortem interval). Purpose : To compare the stages of decay, genus, sequence of arrival, and growth rate of the fly larvae on corpses of rat exposed to open wound and control. Method : This study is using experimental cohort design with exposing open wound on corpses of rat. Result : In control corpses of rat, the initial decay stage (24 hours), putrefaction stage (48-72 hours), stage of active decay (72-96 hours), post decay stage(120 hours), skeletonization stage (144 hours). While on corpses of rat with traumatic an open wound, initial decay stage (24 hours), putrefaction stage (48 hours), active decay stage (48-72 hours), post decay stage(72-96 hours), skeletonization stage (120 hours). The genus of fly larvae found on the control and on the open wound exposed corpses of rat were Calliphora sp., Chrysomya sp., Sarcophaga sp. The sequence of arrival of fly on control and open wound corpses of rat were Calliphora sp., Chrysomya sp., Sarcophaga sp. There are statistically significant mean difference between control and open wound on lenght and weight growth of Chrysomya sp. larvae, weight growth of Calliphora sp. larvae on the third day. Conclusion: Active decay, post-decay, adn skletonization stage on traumatic an open wound exposed corpses 24 hours faster than the control. There are no differences in larvae genus and arrival sequence on traumatic an open wound exposed corpse and control. There are difference in growth of Chrysomya sp., weight of Calliphora sp. larvae between control and traumatic an open wound on the third day. Keyword : Fly Larvae, Open Wound, Post Mortem Inteval

Kata Kunci : Fly Larvae, Open Wound, Post Mortem Inteval