PERBEDAAN EKSPRESI INTERLEUKIN-8 PADA INFLAMASI KRONIK DAN KRONIK AKTIF BIOPSI MUKOSA LAMBUNG DENGAN INFEKSI Helicobacter pylori

MUHAMMAD ARIF, dr. Neneng Ratnasari, SpPD-KGEH; Prof. dr. Siti Nurdjanah, M.Kes., SpPD-KGEH

2015 | Tesis-Spesialis | SP Ilmu Penyakit Dalam

Latar belakang: Infeksi Helicobacter pylori (HP)diperkirakan terjadi pada 50% populasi di dunia. Infeksi Helicobacter pylori menyebabkan inflamasi mukosa lambung dan epitel lambung melepaskan interleukin-1ß, interleukin-6, interleukin-8, dan tumor necrosis factor a. Interleukin-8 berperan dalam derajat inflamasi kronik mukosa lambung dan risiko terjadinya kanker lambung. Belum ada penelitian tentang perbedaan ekspresi interleukin-8 berdasarkan pada inflamasi kronik dan kronik aktif pada biopsi mukosa lambung dengan infeksi Helicobacter pylori di RSUP Dr. Sardjito Tujuan Penelitian: Untuk membuktikan adanya perbedaan ekspresi interleukin-8 pada inflamasi kronik dan kronik aktif biopsi mukosa lambung dengan infeksi Helicobacter pylori. Metode: Penelitian ini menggunakan desain potong lintang. Dilakukan pemeriksaan ekspresi interleukin-8 pada sisa sampel biopsi HP (+) bulan Agustus 2009 sampai Maret 2014. Klasifikasi inflamasi kronik dan kronik aktif diperoleh melalui laporan histopatologi. Data numerik-kategorik dari 2 kelompok, tidak berpasangan, distribusi tidak normal: uji Mann-Whitney U. Perbedaan dianggap bermakna jika didapatkan p< 0,05 dengan interval kepercayaan 95% Hasil: Didapatkan 41 sampel preparat biopsi mukosa lambung, terdiri 9 sampel inflamasi kronik dan 32 sampel inflamasi kronik aktif mukosa lambung. Nilai median ekspresi IL-8 eptitel permukaan pada inflamasi kronik sebesar 83 (4094)% dan pada inflamasi kronik aktif sebesar 82 (19-94). Dilakukan uji komparasi dengan uji Mann Whitney U. Dari perhitungan statistik didapatkan nilai p=0,887 dengan p>0,05. Simpulan: Tidak terdapat perbedaan ekspresi interleukin-8 pada inflamasi kronik dan inflamasi kronik aktif mukosa lambung dengan infeksi helicobacter pylori.

Background: Infection with Helicobacter pylori (HP) is estimated to occur in 50% of the population in the world. Helicobacter pylori infection causes inflammation of the gastric mucosa and gastric epithelial release of interleukin-1ß, interleukin-6, interleukin-8 and tumor necrosis factor a. Interleukin-8 plays a role in the degree of chronic inflammation of the gastric mucosa and gastric cancer risk. There has been no research on differences in the expression of interleukin-8 is based on chronic and chronic active inflammation on biopsy of the gastric mucosa with Helicobacter pylori infection in Hospital Dr. Sardjito. Objective: To prove the existence of differences in the expression of interleukin-8 in chronic inflammation and gastric mucosal biopsy active chronic infection with Helicobacter pylori. Methods: This study used a cross-sectional design. Examined the expression of interleukin-8 on the rest of the biopsy sample HP (+) in August 2009 to March 2014. Classification of chronic inflammatory and chronic active obtained through histopathology report. Categorical numerical data from the two groups, unpaired, the distribution is not normal: Mann-Whitney test. Differences were considered significant if it was obtained p <0.05 with a confidence interval of 95%. Results: There were 41 samples of gastric mucosa biopsy preparations, comprising 9 samples of chronic inflammation and 32 samples of chronic inflammatory active of the gastric mucosa. The median value of the expression of IL-8 eptitel surface on chronic inflammation by 83 (40-94)% and the active chronic inflammation by 82 (19-94). Comparison test with Mann Whitney U test. From the statistical calculations p value = 0.887 with p> 0.05 Conclusion: There is no difference in the expression of interleukin-8 in inflammatory chronic active and chronic inflammation of the gastric mucosa with Helicobacter pylori infection.

Kata Kunci : Helicobacter pylori, interleukin-8, inflamasi kronik dan inflamasi kronik aktif, Helicobacter pylori, interleukin-8, chronic inflammation and chronic inflammatory active