Aktivitas Antibakteri dan Antioksidan Hidrolisat Protein Gonad Lele Betina

IGAN HASUNA ALFIANDRA, Prof. Dr. Ir. Ustadi, M.P; Indun Dewi Puspita, S.P., M.Sc., Ph.D.

2015 | Skripsi | S1 TEKNOLOGI HASIL PERIKANAN

Penelitian ini bertujuan untuk mengetahui ektrak protein telarut, aktivitas antibakteri dan antioksidan dari hidrolisat protein gonad lele betina secara enzimatis. Sampel gonad betina dipreparasi dengan dihomogenkan dengan buffer fosfat dengan perbandingan 1:6. Kemudian disentrifuge 12.000 rpm, 4oC selama 15 menit. Cairan dan pelet dipisahkan, kemudian dilakukan pretisipasi amonium sulfat 20%, 40%, 60%, dan 80%. Masing-masing presipitat didialisis selama 12 jam sebelum dihidrolisis. Presipitat dan dialisat diuji kandungan protein terlarut dan hidrolisat protein diuji antibakteri dengan difusi dan uji antioksidan dengan DPPH. Total protein terlarut presipitat dan dialisat fraksi 20%, 40%, 60%, dan 80% yaitu 4078,93 mg/ml; 6.835,92 mg/ml; 13.033,82 mg/ml; 8.070,85 mg/ml dan 2.315,81 mg/ml; 1.787,14 mg/ml; 2.271,93 mg/ml; 9.853,06 mg/ml; 6.263 mg/ml. Hasil uji antibakteri hidrolisat papain untuk E. coli sebesar 0,64 mm; 2,21 mm; 3,23 mm; 4,80 mm; dan 3,26 mm. Bacillus cereus sebesar 0,64 mm; 2,21 mm; 3,23 mm; 4,80 mm; dan 3,26 mm. Hasil uji antibakteri hidrolisat pepsin untuk E. coli sebesar 1,23 mm; 1,70 mm; 0 mm; 1,89 mm dan 1,31 mm. Bacillus cereus sebesar 1,06 mm; 0 mm; 0,95 mm; 3,08 mm; dan 0 mm. Uji antioksidan tidak menunjukkan adanya aktivitas antioksidan dan sampel hidrolisat protein berperan sebagai oksidator dengan nilai inhibisi negatif.

This research aimed to find out the extraction of disolved protein, antibacterial and antioxidant activites from protein hidrolizate of catfish female gonad through enzymatic process. Female fish gonad is prepared by mixing with buffer phosphate using 1:6 scale. Then, being sentrifuged in 12.000 rpm, in 4oC for 15 minutes. The liquid and solid compound are separated. After that dealing with ammonium sulphate presipitation for 20%, 40%, 60%, and 80%. Every single result of presipitation is processed on dialysis for 12 hours continously before being hidrolized. The finding of precipitation and dialysis are examined on theirs disolved protein. Protein hydrolyzates examined on its antibacteria using disc diffusion test and antioxidant activity using DPPH. Through this process the researcher could find the total of exraction of disolved protein from precipitation and dialysis fraction for 20%, 40%, 60%, and 80% are 4078,93 mg/ml; 6.835,92 mg/ml; 13.033,82 mg/ml; 8.070,85 mg/ml and 2.315,81 mg/ml; 1.787,14 mg/ml; 2.271,93 mg/ml; 9.853,06 mg/ml; 6.263 mg/ml. The antibacterial examination using papain enzyme hydrolysis for E. coli found 0,64 mm; 2,21 mm; 3,23 mm; 4,80 mm; and 3,26 mm. For Bacillus cereus found 0,64 mm; 2,21 mm; 3,23 mm; 4,80 mm; and 3,26 mm. The antibacterial examination using pepsin enzyme hydrolysis for E. coli found 1,23 mm; 1,70 mm; 0 mm; 1,89 mm and 1,31 mm. For Bacillus cereus found 1,06 mm; 0 mm; 0,95 mm; 3,08 mm; dan 0 mm. The antioxidant examination did not show the presence of antioxidant activity and the protein hidrolyzates are known as the oxydator compound following the result of negative inhibition.

Kata Kunci : Kata kunci : antibakteri, antioksidan, dialisis, hidrolisat, total protein