GAMBARAN HISTOPATOLOGI TIROID PADA TIKUS OVARIEKTOMI YANG DIBERI TAMOXIFEN DAN ETINIL ESTRADIOL

ADNAN YOGA HERTANTYO, drh. Rr. Devita Anggraeni, M.P., Ph.D.

2015 | Skripsi | S1 KEDOKTERAN HEWAN

Penggunaan tamoxifen dan etinil estradiol sebagai terapi pengganti estrogen menunjukkan efek beragam pada beberapa organ. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian tamoxifen dan etinil estradiol terhadap histopatologi tiroid tikus Sprague-Dawley dua bulan setelah ovariektomi. Tikus betina berumur +/- 2 bulan sejumlah 16 ekor dibagi menjadi 4 kelompok (kelompok A, B, C dan D), masing-masing 4 ekor, kelompok A yaitu tikus non ovariektomi dengan pemberian aquabides (1 ml/hari), kelompok B yaitu tikus ovariektomi dengan pemberian aquabides (1 ml/hari), kelompok C yaitu tikus ovariektomi dengan pemberian tamoxifen (10 mg/kgBB/hari), dan kelompok D yaitu tikus ovariektomi dengan pemberian etinil estradiol (0,2 mg/kgBB/hari). Seluruh tikus diberi pakan standar serta air minum secara ad libitum. Pemberian obat dilakukan selama dua bulan setelah ovariektomi. Setelah perlakuan selesai, dilakukan nekropsi untuk pengambilan tiroid dan dimasukkan dalam larutan Neutral Buffer Formalin 10%, untuk pembuatan preparat histopatologi dengan pengecatan Hematoxilin-Eosin. Hasil penelitian menunjukkan adanya perbedaan histopatologi tiroid tikus non ovariektomi terhadap tiroid tikus ovariektomi. Secara histopatologi, pada tikus non ovariektomi menunjukkan epitelium berbentuk kuboid dan lumen folikel berisi koloid. Pada tikus ovariektomi yang diberi aquabides menunjukkan epitelium berbentuk skuamus, lumen folikel tampak lebih besar dan berisi koloid. Pada tikus ovariektomi yang diberi tamoxifen maupun etinil estradiol menunjukkan beberapa epitelium berbentuk skuamus, sebagian lumen folikel masih tampak besar dan berisi koloid, dan ada vesikula-vesikula kecil di tepi koloid yang menunjukkan adanya aktivitas kelenjar tiroid. Dengan demikian, berdasarkan gambaran histopatologi, maka dapat disimpulkan bahwa pemberian tamoxifen dan etinil estradiol selama dua bulan pasca ovariektomi dapat memperbaiki aktifitas kelenjar tiroid.

The use of tamoxifen and ethinyl estradiol as estrogen replacement therapy showed diverse effects on several organs. Therefore, this research was conducted to study the effect of tamoxifen and ethinyl estradiol against thyroid histopathologic figure of Sprague-Dawley rats two months after ovariectomy. Sixteen females rats at +/- 2 months of age were divided into 4 groups (groups A, B, C and D), Group A was non-ovariectomized with administration of aquabides (1 ml/day), group B was ovariectomized with administration of aquabides (1 ml/day), group C was ovariectomized with administration of tamoxifen (10 mg/kg/day), and group D was ovariectomized with administration of ethinyl estradiol (0.2 mg/kg/day). Rats were fed standard diet and water was given ad libitum. Treatment was done for two months after ovariectomy. After treatment was completed, necropsy was performed for taking the thyroid and fixed it in Neutral Buffer Formalin 10%. For histopathologic examination using Hematoxilin-eosin staining. The results showed differences in thyroid histopathology of non-ovariectomized rats to ovariectomized rats. Histopathologically, the non-ovariectomized rats showed cuboidal epithelium and lumen of the follicle was contain colloid. In ovariectomized rats with aquabides administration showed squamous epithelium, and larger follicular lumen contains colloids. In ovariectomized rats with tamoxifen and ethinyl estradiol administration showed squamous epithelium, some larger lumen contains colloid were still present, and small vesicles were found at the edge of the colloid which showed activity of the thyroid gland. Thus, based on histopathologic, it can be concluded that the administration of tamoxifen and ethinyl estradiol for two months after ovariectomy can improve the activity of the thyroid gland.

Kata Kunci : Etinil estradiol, ovariektomi, tamoxifen, tiroid.