PENGARUH PROBIOTIK YOGHURT TERHADAP PERTUMBUHAN BAKTERI Aggregatibacter actinomycetemcomitans PENYEBAB PENYAKIT PERIODONTAL (Kajian Secara In Vitro)

ATFIRANI TRI SUKMA, drg. Prayitno, SU.; Dr. drg. Ahmad Syaify Sp.Perio (K)

2015 | Skripsi | S1 PENDIDIKAN DOKTER GIGI

Penyakit periodontal merupakan penyakit yang mengenai jaringan periodontium yang disebabkan oleh bakteri periodontopatogen, salah satunya adalah Aggregatibacter actinomycetemscomitans. Bakteri ini merupakan bakteri gram negatif, memiliki leukotoksin dan endotoksin yang mampu merusak jaringan. Probiotik yoghurt mengandung senyawa antibakteri berupa bakteriosin, asam laktat, dan hidrogen peroksida yang mampu menghambat pertumbuhan bakteri. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh probiotik yoghurt terhadap bakteri Aggregatibacter actinomycetemcomitans secara in vitro. Metode uji zona hambat yang digunakan adalah metode difusi agar yang terdiri atas dua kelompok yaitu kelompok perlakuan (probiotik yoghurt) dan kelompok kontrol (akuades). Masing-masing kelompok terdiri atas 18 sumuran. Setiap cawan petri berisi 6 sumuran yang terdiri dari 3 sumuran untuk kontrol negatif dan 3 sumuran untuk perlakuan. Inkubasi selama 24 jam untuk melihat diameter zona hambat yang terbentuk disekitar sumuran. Data dianalisis menggunakan independent T-test dan uji Levene. Hasil penelitian menunjukkan bahwa pada sumuran yang berisi probiotik yoghurt terdapat zona hambat berupa area bening disekitar sumuran. Terdapat perbedan yang bermakna jika dibandingkan dengan kontrol negatif. Kesimpulan dari penelitian ini adalah probiotik yoghurt mampu menghambat pertumbuhan bakteri Aggregatibacter actinomycetemcomitans.

Periodontal disease is disease of periodontal tissue which is cause by periodontopatogen bacteria. One of them is Aggregatibacter actinomycetemcomitans. The bacteria is a negatif grams, have a leukotoxin and endotoxin which can destruct the tissue. Probiotic yoghurt contain antibacterial compounds like bacteriosin, lactic acid, and hydrogen peroxide which is able to inhibit bacterial growth. The aim of this study is to know the in vitro effect of probiotic yoghurt to Aggregatibacter actinomycetemcomitans. Method of inhibition zone used in this study was agar diffution which consist of two groups : treatment group (probiotic yoghurt) and control group (aquades steril) each groups consist of 18 wells. Each petri dics consist of 6 wells : 3 for negative controls and 3 for treatment groups on petri disc with agar diffution method on petri disc. Twenty four hours incubation was done to observe the diameter of inhibition zone which was formed around the wells. Sterile aquades was used as negative control, The data was analyzed with independent T-test and Levene test. The result of this study showed that there was inhibition zone which was formed on the wells with probiotic yoghurt. Inhibition zone was showed as clear area around the wells. There are significant differences between treatment groups and control groups. It was concluded that probiotic yoghurt was able to inhibit Aggregatibacter actinomycetemcomitans growth.

Kata Kunci : Aggregatibacter actinomycetemscomitans, Probiotik Yoghurt, Penyakit Periodontal