PENGARUH KOMBINASI SUPLEMEN CALCITRIOL DENGAN RALOXIFENE TERHADAP HISTOPATOLOGIS GINJAL TIKUS WISTAR OVARIEKTOMI

HARAKNAS, Dr. drh. Hartiningsih, M.P.

2015 | Skripsi | S1 KEDOKTERAN HEWAN

Suplemen calcitrol dan raloxifene dapat menurunkan risiko patah tulang pada wanita pascamenopause dan kanker kelenjar mamae. Penelitian ini bertujuan untuk mengkaji pengaruh kombinasi suplemen calcitriol dengan raloxifene terhadap gambaran histopatologis ginjal tikus Wistar ovariektomi. Lima belas tikus Wistar betina umur 8 minggu dibagi menjadi tiga kelompok, yaitu kelompok kontrol/non ovariektomi (NK), kelompok ovariektomi (OVK), dan kelompok ovariektomi yang diberi kombinasi suplemen calcitriol 20 ng/hari/tikus dengan raloxifene 3 mg/hari/tikus (OVDR). Masing-masing lima tikus diberi pakan standar dan aquabidestilata secara ad libitum. Tikus ditempatkan dalam kandang individu dengan suhu ruang berkisar 22-250C. Tikus diadaptasikan terhadap pakan dan lingkungan selama 1 minggu. Satu minggu pasca operasi, tikus diberi perlakuan selama 8 minggu. Pada akhir perlakuan tikus di eutanasi, ginjal kiri diambil dan difiksasi dalam formalin 10 % untuk pemeriksaan hitopatologis ginjal dengan pewarnaan HE. Hasil pemeriksaan histopatologis ginjal pada tikus kontrol/non ovariektomi dan tikus ovariektomi tidak menunjukkan perubahan. Pada tikus ovariektomi yang diberi kombinasi suplemen calcitriol dengan raloxifene gambaran histopatologis tidak menunjukan perubahan. Glomelurus dikelilingi oleh podosit (podocytus) pada stratum viseralis, kapsul glomerulus dilapisi oleh epitel skuamus pada stratum parietalis, tubulus kontortus proksimal dilapisi selapis sel epitel kuboid dengan brush border pada lumen tubulus, tubulus kontortus dikelilingi oleh sel epitel berbentuk kuboid tanpa brush border. Berdasarkan hasil penelitian disimpulkan bahwa tikus ovariektomi yang diberi kombinasi suplemen calcitriol dan raloxifene tidak menimbulkan perubahan pada gambaran histopatologi ginjal.

Calcitrol and raloxifene supplemen could reduce the risk of fractures in postmenopausal women and cancer of the mammary gland. The objective of this research is to study the effect of combination calcitriol and raloxifene supplementation on histopathological preview of kidney in ovariectomized Wistar rats. Fifteen female Wistar rats at 8 weeks of age, were divided into three group, control/non-ovariectomized rats (NK), ovariectomized rats (OVK), and ovariectomized rats with calcitriol 20 ng/hari and raloxifene supplements 3 mg/day/rat (OVR), and were fed standard diet, and were given aquabidestilata ad libitum. Each of rats were placed into individual cage in temperature 22-250C. Rats were adapted to feed and the environment for 1 week. One week after surgery, rats were treated for 8 weeks. In the end of treatment rat was euthanized, left kidney was taken and fixated in 10% formalin for kidney histopathology exemination by HE staining. As result, non-ovariectomized/control and ovariectomized rats showed no change in kidney. In ovariectomized rats with combination calcitriol and raloxifene supplementation showed no change in kidney. Glomelurus was surrounded by Podosit (podocytus) in the stratum visceral, Glomerular capsule is lined by squamous epithelium in the stratum parietal, The proximal tubules have cuboidal cells with prominent brush border (microvilli), the distal tubules have cuboid epithelium similar to proximaltubules, but devoid of brush border. Based on the results of the study concluded that the ovariectomized rats were given a combination of calcitriol and raloxifene supplementation did not result in changes in renal histopathology preview.

Kata Kunci : ovariektomi, histopatologis ginjal, calcitriol, raloxifene