PENGARUH PEMBERIAN TAMOXIFEN DAN ALFACALCIDOL TERHADAP BERAT BADAN DAN BERAT HATI TIKUS OVARIEKTOMI
DIAN BEKTI HADI M, drh. Rr. Devita Anggraini, M.P, Ph.D
2015 | Skripsi | S1 KEDOKTERAN HEWANPenelitian ini bertujuan untuk mengetahui pengaruh tamoxifen dan alfacalcidol terhadap berat badan dan berat hati tikus Sprague-Dawley yang diovariektomi. Dua puluh lima tikus Sprague Dawley betina umur 2 bulan, dibagi 5 kelompok masing-masing 5 tikus. Kelompok A dioperasi semu (non ovariektomi), kelompok B dioperasi ovariektomi, kelompok C dioperasi ovariektomi dan diberi tamoxifen (10 mg/kg BB) , kelompok D dioperasi ovariektomi dan diberi alfacalcidol (0,5 µg/kg BB), kelompok E dioperasi ovariektomi dan diberi kombinasi tamoxifen dan alfacalcidol. Pada bulan kedua pasca operasi ovariektomi, tikus ditimbang berat badanya kemudian dinekropsi untuk pengambilan hati. Hasil penelitian menunjukkan bahwa berat badan tikus ovariektomi (242,8 ± 11,5 gram) berbeda signifikan dengan tikus ovariektomi yang diberi tamoxifen (196,2 ± 16,4 gram) dan tikus ovariektomi yang diberi kombinasi tamoxifen dan alfacalcidol (184,2 ± 4,9 gram) (P<0,05). Berat hati kelompok ovariektomi (2,4 ± 0,16 gram) secara signifikan lebih ringan dibandingkan dengan tikus non ovariektomi (2,9 ± 0,32 gram), tikus ovariektomi yang diberi tamoxifen (2,9 ± 0,18 gram), dan tikus ovariektomi yang diberi kombinasi tamoxifen dan alfacalcidol (3,6 ± 0,48 gram) (P<0,05). Kesimpulan,tikus ovariektomi mengalami penurunan berat badan dan peningkatan berat hati, pemberian tamoxifen dan alfacalcidol berpengaruh signifikan terhadap penurunan berat badan dan peningkatan berat hati
This research was conducted to study the effect of tamoxifen and alfacalcidol on body weight and liver weight of ovariectomy rat. Twenty five females Sprague-Dawley rats at 2 months of age were,divided into 5 groups of 5 mice each. Group A was sham operated (non ovariectomy), group B was ovariectomy, group C was ovariectomy given tamoxifen (10 mg/kg BB), group D was ovariectomy given alfacalcidol (0,5 µg/kg BB),, group E was ovariectomy given combination of tamoxifen and alfacalcidol. Two months after ovariectomy the rats were weighed and necropsized to remove the liver. Liver were taken. The results showed that the body weight ovariectomized rats (242,8 ± 11,5 grams) were significantly higher than ovariectomized rats were given tamoxifen (196,2 ± 16,4 grams) and ovariectomized rats were given combination of tamoxifen and alfacalcidol (184,2 ± 4,9 grams) (P<0,05). Liver weight of ovariectomized rats (2,4 ± 0,16 grams) were significantly lighter than non ovariectomized rats (2,9 ± 0,32 grams), ovariectomized rats were given tamoxifen (2,9 ± 0,18 grams) and ovariectomized rats were given combination of tamoxifen and alfacalcidol (2,9 ± 0,18 grams) (P<0,05). Based on the result, ovariectomized rats were ovariectomized rats had decreased body weight and increase liver weight. Ovariectomized rats were given combination of tamoxifen and alfacalcidol had decreased body weight and increase liver weight.
Kata Kunci : ovariektomi , hati, tamoxifen, alfacalcidol
