Perbandingan Pengaruh Aloe Vera, Madu, Saliva dan Putih Telur Terhadap Ekspresi Sel T CD4+ Pada Luka Pasca Insisi Kulit Tikus
LAUHIL MAHFUDZ, dr. Ishandono Dachlan,M.Sc,Sp.B.,Sp.BP-RE(K); Dra. Dewajani Purnomosari, M.Si., Ph.D
2015 | Tesis | SP ILMU BEDAHPendahuluan: Sel T CD4+ berperan penting dalam regulasi penyembuhan luka (Davis P.A, Br J Surg.Feb;88(2):298-304.2001). CD4+ merupakan upregulator of wound healing.(Boyce D.E, Aidline national library of medicine. Jul;143 (1): 59-65.2000) .Peningkatan CD4+ dapat menggambarkan perbaikan proses penyembuhan luka. Hipotesis, ekspresi sel T CD4+ pada kelompok perlakuan luka dengan bahan-bahan tersebut lebih tinggi dibandingkan dengan kelompok kontrol. Tujuan: Membandingkan ekspresi sel T CD4+ dalam proses penyembuhan luka pada pemberian topikal aloe vera, madu, saliva manusia, putih telur, dan moist dressing sebagai kontrol. Metode: Dilakukan penelitian laboratorik, pada 25 ekor tikus Wistar (Rattus novergicus) dibagi 5 kelompok, 4 kelompok diberikan bahan uji aloe vera, madu, saliva manusia, putih telur, dan 1 kelompok kontrol diberikan NaCl 0,9%. Setelah anestesi umum, dibuat luka insisi dipunggung, luka dijahit, dilakukan pengolesan dengan bahan uji, perlakuan dressing dan bahan uji diberikan 2 kali sehari selama 6 hari. Pada hari ke 7 dilakukan euthanasia, jahitan dilepas, kulit bekas insisi diambil dan kemudian dilakukan pemeriksaan imunohistokimia, hitung dan analisis ekspresi sel T CD4+. Hasil: Dari hasil penelitian didapatkan rerata skor histologis kelompok perlakuan bahan uji lebih tinggi daripada kelompok kontrol. skor histologis kelompok madu 4804.15 ± 979.19, aloe vera 4550.30 ± 1408.04, putih telur 4703.20 ± 3381.21, saliva 2356.77 ± 1069.07, dan NaCl 1565.96 ± 560.00. Terdapat perbedaan bermakna skor histologis madu dengan NaCl 0,9% (p-value = 0.009 < 0.05), aloe vera dengan NaCl 0,9% (p-value = 0.009 < 0.05), dan putih telur dengan NaCl 0,9% (p-value = 0.028 < 0.05). Tidak terdapat perbedaan bermakna skor histologis saliva dengan NaCl 0,9% (p-value = 0.175 > 0.05). Kesimpulan: Luka insisi kulit yang diberi bahan uji topikal madu, aloe vera, dan putih telur ekspresi sel T CD4+ secara bermakna lebih tinggi dibandingkan dengan kontrol. Nilai skor histologis madu paling tinggi dibandingkan bahan uji lainnya.
Background: T CD4+ cell has important role in wound healing regulation (Davis P.A, Br J Surg.Feb;88(2):298-304.2001). CD4+ plays role as upregulator of wound healing.(Boyce D.E, Aidline national library of medicine. Jul;143 (1): 59-65.2000). Increased number of CD4+ cell around the tissue could described the repair process of wound healing. Hypothesis of this study, T CD4+ cell expression in groups that are applicated with experimental substrates are higher comparing to control group. Objective: The study aim is to compare T CD4+ cell expresion in wound healing process on application of Aloe vera, Honey, Saliva, White egg, dan moist dressing as control. Methods: This laboratoric experimental study on 25 Wistar rats (Rattus novergicus) are devided into 5 groups. 4 groups are given topical application with Aloe vera, Honey, Human saliva, White egg, dan 1 group as control with NaCl 0,9%. We performed general anaesthesia procedure, make incision wound on skin of back, then the wound was sutured, on surface of the sutured wound we applicated with substrate twice a day as long as 6 days. On day 7, we performed euthanasia, the suture is cutted off, the incised skin is taken for immuno histochemistry examination, we counted and analized the T CD4+ cell expression. Result: Mean histologic score for groups which had received application with experimental substrate is higher compared to control group. Histologi score for honey is 4804.15 ± 979.19, aloe vera is 4550.30 ± 1408.04, white egg is 4703.20 ± 3381.21, saliva is 2356.77 ± 1069.07, dan NaCl is 1565.96 ± 560.00. we find there is difference of histologic score between honey and NaCl 0,9% (p-value = 0.009 < 0.05), aloe vera and NaCl 0,9% (p-value = 0.009 < 0.05), also white egg and NaCl 0,9% (p-value = 0.028 < 0.05). But there is no difference of histologic score between saliva and NaCl 0,9% (p-value = 0.175 > 0.05). Conclusion: The T CD4+ cell expression in incised wound skin which are applicated with experimental substrate Honey, Aloe vera, and White egg significantly higher compared to NaCl 0,9% as control.
Kata Kunci : penyembuhan luka, aloe vera, madu, saliva, putih telur, ekspresi sel T CD4+
