EFEK SIPROFLOKSASIN TERHADAP DEGRADASI KOLAGEN DAN PROLIFERASI FIBROBLAS KELOID

THIANTI SYLVININGRUM, Dr. dr. Yohanes Widodo Wirohadidjojo, Sp.KK(K)

2015 | Tesis | S2 KEDOKTERAN KLINIK/MS-PPDS

Keloid merupakan tumor dermis jinak yang ditandai peningkatan proliferasi fibroblas dan sintesis kolagen. Siprofloksasin telah terbukti secara in vitro menyebabkan kerusakan tendon dan sendi melalui penurunan proliferasi fibroblas serta peningkatan degradasi kolagen melalui peningkatan aktivitas MMP, tetapi efeknya terhadap fibroblas keloid belum diketahui. Penelitian ini bertujuan menilai efek siprofloksasin terhadap degradasi kolagen dan proliferasi fibroblas keloid. Biakan fibroblas keloid dibagi menjadi 4 kelompok yaitu 1 kelompok kontrol dan 3 kelompok perlakuan yang masing-masing diberi siprofloksasin 2,5 μg/ml, 10 μg/ml, dan 40 μg/ml selama 48 jam dengan pengulangan masingmasing kelompok sebanyak 3 kali. Rerata densitas optik kolagen terdegradasi dinilai menggunakan sirius red sedangkan MTT assay digunakan untuk menilai densitas optik proliferasi fibroblas. Hasil kedua pengukuran tersebut dibaca menggunakan spektrofotometer dengan panjang gelombang 570 nm. Analisis data perbandingan rerata densitas optik kolagen terdegradasi menggunakan uji one way ANOVA, sedangkan rerata densitas optik proliferasi fibroblas dianalisis menggunakan uji Friedman, dilanjutkan uji Wilcoxon. Hasil penelitian ini menunjukkan bahwa tidak ada perbedaan yang signifikan pada rerata densitas optik kolagen terdegradasi dan proliferasi sel, antara kelompok fibroblas keloid terpapar siprofloksasin dan kelompok kontrol (p>0,05). Hasil penelitian ini dapat disimpulkan bahwa siprofloksasin tidak menunjukkan efek pada peningkatan kolagen terdegradasi dan penurunan viabilitas sel fibroblas keloid. Kata kunci : siprofloksasin, fibroblas, kolagen, keloid

Keloid is a benign dermal tumor characterized by increase in the proliferation of fibroblasts and collagen synthesis. Ciprofloxacin has been known to cause damage tendons and joints in vitro through a decrease in fibroblast proliferation and increase collagen degradation due to the increase of MMP activation, however its effect on keloid fibroblasts still remains unknown. Therefore, we conducted a study to assess the effect of ciprofloxacin on collagen degradation and proliferation of keloid fibroblasts. Keloid fibroblast cultures were divided into 4 groups, 1 control group and 3 treatment groups which were exposed to ciprofloxacin 2,5 ug/ml, 10 ug/ml, and 40 ug/ml for 48 hours with 3 times repetition for each group. The mean optical density of degraded collagen was analyzed using sirius red, while MTT assay was performed to analyze keloid fibroblast cell proliferation. The results were measured by a spectrophotometer with 570 nm wavelength. The mean difference of optical density of degraded collagen was analyzed using one way ANOVA test, while the mean difference optical density of keloid fibroblast cell proliferation was analyzed using the Friedman test followed by Wilcoxon test. The mean optical density of degraded collagen and fibroblast cell proliferation of ciprofloxacin-treated groups were not significantly different compare to control group (p>0,05). Therefore, we concluded that ciprofloxacin has no effect on the increase of collagen degradation and decrease of keloid fibroblast cell proliferation. Keywords : ciprofloxacin, fibroblast, collagen, keloid

Kata Kunci : siprofloksasin, fibroblas, kolagen, keloid; ciprofloxacin, fibroblast, collagen, keloid