PENGARUH PENGGUNAAN EKSTRAK ETANOLIK Sargassum polycystum TERHADAP PROFIL BIOKIMIA DARAH TIKUS WISTAR STRES

RR. RADIPTA LAILATUSSIFA, Dr. Amir Husni, S.Pi., M.P. ; Prof.Dr. Agung Endro Nugroho, M.Si.,Apt.

2014 | Skripsi | TEKNOLOGI HASIL PERIKANAN

Intisari Penelitian ini bertujuan untuk mengetahui adanya kandungan polifenol ekstrak Sargassum polycystum serta efek ekstrak polifenol Sargassum polycystum dalam mempengaruhi profil kadar glukosa, trigliserida, kolesterol, ALP, SGPT, SGOT, dan gastric ulcer sebagai faktor yang diduga berpengaruh penting terhadap stres oksidatif. Polifenol diekstrak menggunakan ethanol 96% kemudian diuji kandungan total fenol senyawanya serta analisis kualitatif menggunakan TLC dan HPLC. Tikus Wistar betina usia 3 bulan dengan berat 150-170 gram sebanyak 25 ekor dibagi menjadi 5 kelompok yaitu kontrol normal (sehat), kontrol negatif (stres tanpa obat), dan kontrol positif (stres+diazepam 0,18 mg/kg; stres+polifenol Sargassum polycystum 150 mg/kg; stres+polifenol Sargassum polycystum 450 mg/kg). Kelompok kontrol negatif dan positif diberi perlakuan stres dingin dengan cara mencelupkan tikus ke dalam air es suhu 7oC selama 10 menit. Stresor diberikan sehari sekali selama 14 hari. Berat badan masing-masing tikus diukur setiap hari. Darah tikus diambil 1,5 cc pada Plexus Retroorbitalis sebanyak 3x, yaitu hari ke-0 (sebelum diberi stres), hari ke-7, dan hari ke-14 guna analisis glukosa, TGA, kolesterol, ALP, SGPT, dan SGOT. Hari ke-14, tikus dimatikan dan diambil lambungnya untuk uji gastric ulcer. Kandungan total fenol ekstrak Sargassum polycystum sebesar 59,3 g GAE/100 g ekstrak. Ekstrak Sargassum polycystum mengandung polifenol berupa asam galat dan fenol berdasarkan uji HPLC. Ekstrak polifenol Sargassum polycystum 450 mg/kg memiliki kemampuan terbaik dalam mencegah terjadinya stres oksidatif ditunjukkan dengan tidak adanya ulkus lambung pada tikus; penurunan kadar glukosa darah sewaktu, peningkatan kadar TGA, penurunan kadar kolesterol, dan penurunan kadar enzim ALP, SGPT, serta SGOT. Kata kunci: gastric ulcer, polifenol, Sargassum polycystum, stres dingin, stres oksidatif

Abstract The aim of the research was to know contents of Sargassum polycystum polyphenols extract and effects of Sargassum polycystum polyphenols extract within influenced value profil of glucose, triglyceride, cholesterol, ALP, ALT, AST, and gastric ulcers as factors that expected had important influences to oxidative stress. Polyphenols was extracted using 96% ethanol then tested total phenolic contents of the extract and qualitative analysis used TLC and HPLC. Female Wistar rats aged 3 months, weight 150-170 gram as many as 25 tail divided into 5 group, that were normal control, negative control, and positive control (stress+0,18 mg/kg diazepam; stress+150 mg/kg polyphenols of Sargassum polycystum; and stress+450 mg/kg polyphenols of Sargassum polycystum). Negative and positive control groups gave cold restraint stress by getting dipped rats in the ice water with temperature of 7oC during 10 minutes. Stressor gave once a day until 14 days. Weights of each rats measured every day. Rats blood took about 1,5 cc in Plexus Retroorbitalis 3 times, on days-0, days-7, and days-14 for analysis of glucose, triglyceride, cholesterol, ALP, ALT, and AST. 14th days, rats be dead and took the gastric for gastric ulcer analysis. TPC of Sargassum polycystum extract was 59,3 g GAE/100 g extract. Sargassum polycystum extract contains polyphenols such as galic acids and phenols grounded on HPLC test. Polyphenols of Sargassum polycystum extract with 450 mg/kg concentrations had best capability within prevent oxidative stress that shown nothing gastric ulcers, increased of triglyceride values, decreased of in time blood glucose, cholesterol, ALP, ALT, and AST values. Keywords: cold restraint stress, gastric ulcers, oxidative stress, polyphenols, Sargassum polycystum

Kata Kunci : cold restraint stress, gastric ulcers, oxidative stress, polyphenols, Sargassum polycystum