STUDI PELEPASAN IN VITRO KURKUMIN DARI BEADS KALSIUM PEKTIN KITOSAN DENGAN PENAMBAHAN PENGEMULSI ASAM STEARAT

ARIESTA SETYA NINGRUM, Prof. Drs. MUDASIR, M.Eng., Ph.D.; Drs. DWI SISWANTA, M.Eng., Ph.D.

2014 | Skripsi | KIMIA

STUDI PELEPASAN IN VITRO KURKUMIN DARI BEADS KALSIUM-PEKTIN-KITOSAN DENGAN PENAMBAHAN PENGEMULSI ASAM STEARAT Ariesta Setya Ningrum 10/300352/PA/13321 INTISARI Telah dilakukan penelitian tentang laju pelepasan kurkumin dari beads kalsium pektin kitosan (Ca-pektin kitosan) dengan penambahan asam stearat sebagai pengemulsi. Penelitian ini bertujuan untuk mengetahui efektivitas beads yang telah ditambah dengan asam stearat dalam pelepasan kurkumin di dalam larutan buffer:etanol (v/v). Dalam penelitian ini telah dipelajari pengaruh komposisi kurkumin, komposisi kitosan, komposisi asam stearat, komposisi etanol dan variasi pH medium pelepasan terhadap laju pelepasan kurkumin. Beads pektin teremban kurkumin yang mengandung emulsifier asam stearat dibuat dengan menggunakan larutan kalsium klorida (CaCl2) sebagai agen pembentukan beads pektin yang kemudian disalut dengan larutan kitosan. Beads kemudian dikarakterisasi dengan spektrometer IR. Laju pelepasan kurkumin dari beads diteliti secara in vitro selama 6 jam yaitu dengan merendam beads teremban kurkumin di dalam larutan yang mengandung etanol, kemudian hasil rendaman dianalisis dengan spektrofotometer UV-Vis. Kinetika pelepasan kurkumin dikaji dengan persamaan laju orde nol, orde satu, model Higuchi dan model Korsmeyer-Peppas. Karakterisasi dengan FTIR beads Ca-pektin kitosan dengan pengemulsi asam stearat menunjukkan bilangan gelombang spesifik pada 3425 cm-1 yang merupakan gugus hidroksi, 2916 cm-1 dan 2846 cm-1 yang menunjukkan C–H sp3 pada asam stearat. Beads Ca-pektin kitosan yang telah dibuat berdiameter sekitar 0,1667 cm. Hasil penelitian menunjukkan bahwa pelepasan kurkumin dipengaruhi oleh komposisi kurkumin yang termuat, komposisi kitosan penyalut, komposisi asam stearat pada beads dan pH medium pelepasan. Laju pelepasan kurkumin selama 6 jam semakin lambat dan pelepasan semakin sedikit dengan penambahan asam stearat, beads tanpa asam stearat melepaskan 77,91% sedangkan dengan penambahan asam stearat 75 mg atau 1,5% dari komposisi beads dapat melepaskan kurkumin sebesar 18,81% selama 6 jam. Model farmakokinetik menunjukkan bahwa pelepasan kurkumin dari beads mengikuti persamaan Korsmeyer-Peppas dengan laju (k) sebesar 0,0999 menit0,1476 dan mekanisme pelepasan kurkumin didominasi oleh faktor difusi. Kata kunci: beads pektin, kitosan, asam stearat, pelepasan kurkumin

IN VITRO CONTROLLED RELEASE STUDY OF CURCUMIN FROM CALCIUM-PECTIN-CHITOSAN BEADS USING STEARIC ACID AS AN EMULSIFIER Ariesta Setya Ningrum 10/300352/PA/13321 ABSTRACT The controlled release of curcumin from calcium pectin chitosan (Ca-pectin chitosan) beads that emulsified using stearic acid had been studied. The aim of this research was to identify the affectiveness of curcumin released in a buffer: ethanol solution (v/v) from the beads which had been added stearic acid. The research has studied the effect of the amount of loaded curcumin, the amount of chitosan, the amount of stearic acids, amount of ethanol and the variation of the medium pH toward the rate release of curcumin. Curcumin-loaded pectin beads which contained stearic acids has been prepared using calcium chloride (CaCl2) as beading medium then it was coated by chitosan. The beads were characterized using IR spectrometer. In vitro study of controlled release rate of the curcumin from the beads were investigated for 6 hours by soaking the currcumin-loaded beads in the ethanol solutions and the released curcumin was measured by UV-Vis spectrophotometer. The kinetics of releasing curcumin was analyzed by pharmacokinetics models such as zeroth order, first order, Higuchi and Korsmeyer-Peppas models. IR spectrometric characterization of Ca-pectin chitosan beads with emulsifier stearic acid showed absorbtion bands at 3425 cm-1 as specific bands of hydroxyl groups, 2916 cm-1 and 2846 cm-1 showed C–H sp3 groups of stearic acid. Ca-pectin chitosan beads prepared in this study, have diameter of 0.1667 cm. The result of this study showed that the releasing of curcumin was influenced by the amount of loaded curcumin, the amount of chitosan coated, the amount of stearic acid in the beads and pH of the release medium. The rate of releasing curcumin during 6 hours showed that increasing addition of stearic acid decrease the rate of the release. For example, beads without stearic acid releases 77.91% curcumin whereas the beads added 75 mg or 1.5% of the total composition could releases 18.81% during 6 hours. The pharmacokinetics model shows that the curcumin release from the beads followed a Korsmeyer-Peppas model with rate constant (k) of 0.0999 minutes0.1476 and the release mechanism was mainly dominated by diffusion process. Keywords: pectin beads, chitosan, stearic acid, curcumin release

Kata Kunci : pectin beads, chitosan, stearic acid, curcumin release