EFEK ANTELMINTIK Trichoderma sp. DAN FERMENTASINYA PADA HIJAUAN DAUN POTENSI ANTELMINTIK TERHADAP INFEKSI Haemonchus contortus

Drh. Pramu, Dr. drh. R Wisnu Nurcahyo

2014 | Tesis | S2 Sain Veteriner

Pengendalian Haemonchus contortus secara efektif dibutuhkan untuk kesehatan ruminansia kecil. Resistensi antelmintik direspon dengan upaya penemuan bahan alami yang lebih ramah lingkungan. Tujuan penelitian adalah untuk mengetahui efek antelmintik Trichoderma sp. terhadap jumlah egg per gram feses cacing H. contortus dan gambaran darah pada kambing yang terinfeksi H contortus serta fermentasinya pada hijauan daun potensi antelmintik. Trichoderma sp diisolasi dan diperbanyak menggunakan media jagung. Eampat macam hijauan daun potensi antelmintik dikeringkan dengan dijemur untuk kemudia difermentasikan. 15 ekor kambing digunakan dalam uji in vivo dikelopokkan menjadi 5 kelompok; tidak diobati, 5X106 Trichoderma sp, fermentasi hijauan potensi antelmintik Trichoderma sp, hijauan potensi antelmintik, Levamisol. Terdapat perbedaan nyata (P<0,05) penurunan jumlah telur cacing terutama antara kelompok yang tidak diobati dengan kelompok perlakuan. Jumlah eritrosit terdapat perbedaan yang nyata (P<0,05) antara kelompok perlakuan maupun lama waktu pemberian perlakuan. Jumlah leukosit terdapat perbedaan yang nyata (P<0,05) antara lama waktu pemberian perlakuan namun tidak berbeda nyata antar kelompok perlakuan. Konsentrasi Hb menjukkan tidak terdapat perbedaan yang nyata (P>0,05) antara kelompok perlakuan maupun lama waktu pemberian perlakuan. Nilai PCV menjukkan terdapat perbedaan yang nyata (P<0,05) antara kelompok perlakuan maupun lama waktu pemberian perlakuan. Nilai TPP menjukkan terdapat perbedaan yang nyata (P<0,05) antara lama waktu pemberian perlakuan namun tidak berbeda nyata antar kelompok perlakuan. Jumlah eosinofil menjukkan terdapat perbedaan yang nyata (P<0,05) antar kelompok perlakuan namun tidak berbeda nyata antara lama waktu pemberian perlakuan. Kadar selulosa, protein, dan tanin pada daun potensi antelmintik sebelum dan sesudah fermentasi Trichoderma sp berbeda nyata (P<0.05). Pemberian hijauan daun potensi antelmintik, Trichoderma sp, dan fermentasi Trichoderma sp pada kambing yang terinfeksi H contortus masing-masing secara nyata mampu menurunkan jumlah telur cacing H contortus serta meningkatkan jumlah eritrosit dan PCV. Upaya peningkatan potensi antelmintik hijauan daun menggunakan fermentasi Trichoderma sp. tidak terbukti secara signifikan. Fermentasi Trichoderma sp. pada daun potensi antelmintik meningkatkan secara signifikan kadar protein namun menurunkan kadar tanin dan selulosa hijauan. Kata kunci: Trichoderma sp., H contortus, fermentasi, hijauan, antelmintik.

Control to Haemonchus contortus effectively needed for small ruminant health. Anthelmintic resistance responded to the effort of finding natural ingredients that are more environmentally friendly. The purpose of the study was to determine the anthelmintic effect of Trichoderma sp. against the number of eggs per gram feces H. contortus and blood picture in goats that infected with H contortus and fermentation of forages on the potential anthelmintic. Trichoderma sp were isolated and cultivated using corn media. Four kinds of forage potential anthelmintic dried in the sun for later fermented. 15 goats used in vivo test take into 5 groups; Untreated, Trichoderma sp 5X106, fermented forage anthelmintic potential of Trichoderma sp, forage potential anthelmintic, Levamisole. There were significant differences (P <0.05) decrease in the number of worm eggs, especially between the untreated group to the treatment group. The largest decrease occurred in the administration of levamisole, followed by the administration of anthelmintic potential forage, forage fermentation, and the administration of 5X106 Trichoderma sp. There is significant differences the number of erythrocytes (P <0.05) between the treatment groups and the time of treatment duration. On total leukocyte there is significant differences (P <0.05) between the length of time giving treatment but was not significantly different between treatment groups. Hb concentration showed there is no significant difference (P> 0.05) between the treatment group and the time of treatment duration. PCV value showed there is significant differences (P <0.05) between the treatment groups and the time of treatment duration. The value of TPP showed there is significant differences (P <0.05) between the time of treatment duration but was not significantly different between treatment groups. The number of eosinophils showed there are significant differences (P <0.05) between treatment groups, but not significantly different between treatments duration. Levels of cellulose, proteins, and tannins in forage before and after Trichoderma sp fermentation have significantly different (P <0.05). Administration of anthelmintic potential forage, Trichoderma sp, and Trichoderma sp fermentation in goats infected with H contortus respectively significant able to reduce the amount of H contortus worm eggs and increase the number of erythrocytes and PCV. Efforts to increas the potential anthelmintic used forage fermentation of Trichoderma sp. not proven significantly. Fermentation of Trichoderma sp. on leaves anthelmintic potential to significantly increase the protein levels but decrease the tannins and cellulose levels. Keywords: Trichoderma sp., H contortus, fermentation, forage, anthelmintic.

Kata Kunci : Trichoderma sp., H contortus, fermentasi, hijauan, antelmintik.