PELEPASAN KURKUMIN DARI NANOPARTIKEL KITOSAN-KARBOKSIMETIL SELULOSA-GLUTARALDEHIDA

PRAPTANTI SINUNG A.N, Dr. Dwi Siswanta, M.Eng.

2014 | Tesis | S2 Ilmu Kimia

Dalam penelitian ini telah dipelajari kinetika pelepasan kurkumin pada nanopartikel kitosan-karboksimetil selulosa ditaut silang dengan glutaraldehida. Identifikasi gugus fungsi ditentukan dengan metode spektrofotometri Fourier Transform Infrared (FTIR). Sifat kristalinitas diuji dengan X-Ray Diffraction (XRD). Penentuan ukuran partikel dilakukan menggunakan Transmission Electron Microscopy (TEM), sedangkan karakterisasi morfologi diuji dengan Scanning Electron Microscopy (SEM). Studi pelepasan kurkumin ditentukan dengan spektrofotometer UV-Vis. Pada penelitian ini dipelajari pengaruh konsentrasi kitosan, karboksimetil selulosa (KMS), dan glutaraldehida (GA). Model kinetika orde nol, orde satu, Higuchi, dan Korsmeyer-Peppas digunakan untuk menguji kinetika pelepasannya. Hasil penelitian menunjukkan bahwa interaksi elektrostatik antara gugus amina dengan gugus karboksil dapat menyebabkan pergeseran serapan nanopartikel kitosan-KMS-GA pada bilangan gelombang1589 cm -1 . Nanopartikel memiliki ukuran ±70 nm. Komposisi nanopartikel kitosan-KMS-GA optimal diperoleh pada rasio 3:1:0,8. Model pelepasan kurkumin mengikuti kinetika Korsmeyer-Peppas dengan mekanisme difusi Fickian. Pelepasan kurkumin sebanyak 53,82% dengan konsentrasi 1,39 mg/L terjadi pada 5 jam.

The releasing kinetic of curcumin in chitosan-carboxymethyl cellulose nanoparticles crosslinked with glutaraldehyde has been studied. Identification of functional groups was determined by Fourier Transform Infrared (FTIR) spectrophotometry method. The crystallinity characteristic was tested by X-Ray Diffraction (XRD). Particle size determination was performed using Transmission Electron Microscopy (TEM), whereas the morphological characterization was tested by Scanning Electron Microscopy (SEM). Studyof curcumin release was determined by UV-Vis spectrophotometer. In this research, the effects of chitosan, KMS, and glutaraldehyde (GA) concentration were studied. Kinetics model of zero order, first order, Higuchi, and Korsmeyer-Peppas were used to test the kinetics of curcumin release. The results showed that the electrostatic interaction between the amine group with a carboxyl group can cause a shift in the uptake of chitosan-KMS-GA nanoparticles at wave number 1589 cm -1 . Nanoparticles have a size of ±70 nm. The composition of chitosan-KMS-GA nanoparticles concentration obtained at the optimum ratio 3:1:0.8. Model equation of curcumin release followed Korsmeyer-Peppas kinetics with the Fickian diffusion mechanism. Curcumin release of 53.82% as much happened for 5 hours.

Kata Kunci : nanopartikel, kitosan, KMS, glutaraldehida, pelepasan kurkumin