STRUKTUR HISTOLOGIS ORGAN EKSKRETORIK PADA ULAR JALI (Ptyas mucosa Linnaeus, 1758) dan ULAR BELANG HITAM (Pseudolaticauda semifasciata Reinwardt, 1837)

OCTAVIA KHARISMA REMBULAN, Drs. Johanes Sugiyanto, MS

2014 | Skripsi | BIOLOGI

Osmoregulasi adalah proses untuk menjaga keseimbangan konsentrasi air dan elektrolit dalam tubuh. Habitat yang berbeda memiliki karakteristik lingkungan yang berbeda. Penelitian ini akan mengkaji struktur histologis ren, ureter, dan kloaka pada ular jali Ptyas mucosa dan ular belang hitam Pseudolaticauda semifasciata yang memiliki habitat berbeda. Ular jali mewakili ular terestrial, sedangkan ular belang hitam mewakili ular laut. Ular yang digunakan adalah ular betina dewasa. Panjang total tubuh dan berat badan kedua ular diukur. Ular dieuthanasi dengan inhalasi kloroform. Organ-organ ekskresi difiksasi dalam neutral buffered formalin 10% selama ± 24 jam. Pembuatan preparat dilakukan dengan metode parafin dan pewarnaan Hematoksilin Eosin. Preparat histologis ren anterior, ren median, ren posterior, ureter anterior, ureter posterior, dan kloaka diamati dengan mikroskop cahaya. Data yang diperoleh berupa data perbandingan morfologis dan struktur histologis ren, ureter, dan kloaka, serta histomorfometri ren dari kedua spesies ular. Histomorfometri dianalisis menggunakan Student t-Test. Hasil menunjukkan bahwa ren ular belang hitam memiliki glomerulus dengan diameter lebih kecil dan jumlah lebih sedikit dibandingkan ular jali. Tubulus proksimal ular belang hitam memiliki diameter yang lebih kecil dan sel epitelium lebih tinggi dibandingkan dengan ular jali. Diameter ureter ular jali lebih besar dibanding ureter ular belang hitam. Epitelium ureter ular jali transisional, sedangkan epitelium ureter ular belang hitam terlihat kolumnar selapis. Kesimpulan dari penelitian ini yaitu terdapat perbedaan pada struktur histologis ren dan ureter antara ular jali dan ular belang hitam. Struktur histologis kloaka antara kedua ular tidak berbeda.

Osmoregulation is process to maintain balance of water and electrolytes concentration inside body. Each habitat has its own characteristic of environmental conditions. This research was conducted to examine the histological structure of ren, ureter, and kloaka of Common Rat Snake Ptyas mucosa and Black-banded Sea Krait Pseudolaticauda semifasciata. Common Rat Snake represented terrestrial snake whereas Black-banded Sea Krait represented marine snake. Total length and body weight of both snakes (female adult size) was measured. Snakes were euthanized with chloroform inhalation. Ren, ureter, and kloaka were submerged into fixative solution (neutral buffered formaline 10%). Slides were made with paraffin method and Hematoxylin Eosin staining. All slides include ren anterior, ren median, ren posterior, ureter anterior, ureter posterior, and cloaca were observed with light microscope. Morphology and histological structure of ren, ureter, and kloaka of P.mucosa and P.semifasciata were compared. Histomorphometry of ren of both snakes were also measured and analyzed with Student t-Test. The results showed that Black-banded Sea Krait had fewer glomerulus, smaller diameter of glomerulus, smaller diameter of tubulus proximal, and higher epithelium cell of tubulus proximal than those of Common Rat Snake. Common Rat Snake had a bigger ureter than that of Black-banded Sea Krait. Ureter epithelium of Common Rat Snake were transitional, whereas ureter epithelium of Black-banded Sea Krait were simple columnar. Conclusion of this reserach were histological structure of ren and ureter between Common Rat Snake and Black-banded Sea Krait were different, whereas histological structure of cloaca between those two snakes were not different.

Kata Kunci : Organ ekskretorik, struktur histologis, Ptyas mucosa, Pseudolaticauda semifasciata