PENGARUH UKURAN OVARIUM SAPI TERHADAP KUALITAS MORFOLOGI OOSIT YANG DIKOLEKSI SECARA IN VITRO

PUGUH BUDI SUSILO, drh. Agung Budiyanto, M.P., Ph.D.

2014 | Skripsi | KEDOKTERAN HEWAN

Produksi embrio secara in vitro yang terdiri dari maturasi, fertilisasi, dan kultur memerlukan oosit berkualitas agar menghasilkan embrio yang baik. Ovarium sebagai penghasil oosit yang diperoleh dari Rumah Potong Hewan (RPH) memiliki ukuran yang beragam tiap individu. Ovarium yang diperoleh dari Rumah Potong Hewan perlu dilakukan penanganan yang cepat dan tepat untuk keperluan koleksi oosit. Pemilihan oosit secara tidak langsung dapat dilakukan dengan memeriksa ovarium yang mungkin berhubungan dengan kualitas oosit, dalam penelitian ini memeriksa ukuran ovarium. Tujuan penelitian ini adalah untuk mengetahui pengaruh ukuran ovarium sapi terhadap kualitas morfologi oosit. Ovarium yang diambil dari RPH dicuci dengan 0,9% NaCl fisiologis yang sudah ditambah dengan antibiotik Penicillin 100 IU/ml dan Streptomycin 100 μg/ml. Ovarium kemudian dikelompokkan sesuai ukurannya. Kelompok I 1,0 – 1,9 cm, kelompok II 2,0 – 2,9 cm, dan kelompok III 3,0 – 3,9 cm. Koleksi oosit memakai metode aspirasi menggunakan spuit 10 ml dan jarum 18 G. Pengamatan terhadap hasil koleksi oosit dilakukan dengan mikroskop stereo dan dikelompokan berdasarkan kualitas morfologi oosit. Data yang diperoleh dianalisis menggunakan Anova. Hasil penelitian diperoleh bahwa kualitas morfologi oosit tidak memiliki perbedaan (P>0,05) terhadap berbagai ukuran ovarium. Kelompok II menghasilkan oosit dengan kualitas A 34,25% dan B 41,10% lebih banyak daripada kelompok I (22,33% dan 43,69%) dan kelompok III (16,53% dan 32,23%), sedangkan untuk jumlah kualitas oosit A dan B lebih banyak pada kelompok II (75,35%). Data yang diperoleh dapat disimpulkan bahwa ukuran ovarium tidak berpengaruh pada persentase kualitas morfologi oosit A dan B.

The production of embryos using in vitro method which consists of maturation, fertilisationand culture needs qualified oocytes in order to get good embryos. The size of ovarian, the producer of oocytes, which is taken from abbatoir (RPH) is vary from one individu to another. To fulfill the need of oocytes from the abattoir (RPH), ovarian as the primary material required precise and rapid handling. The indirect selection of oocytes could be conduct by examining other organs which have relation to the oocytes quality, and in this research examining the size of the ovarian was the selected way. The purpose of this research was to find out the effects of the size of cow’s ovarian to the quality of oocytes morphology. The ovarians taken from abattoir (RPH) were washed using 0,9% phyciological Nacl which had been added with antibiotics Penicilin 100 IU/ml and Streptomycin 100 μg/ml. Then, the ovarians were classified based on their size. The first group consists of1,0 – 1,9 cm, the second group consists of 2,0 – 2,9 cm and the third group consists of 3,0 – 3,9 cm. Oocytes were collected using aspiration method which used 10 ml spuit and 18 G needle. The observation of the oocytes collection was conduct using stereo microscope and were classified based on the oocytes morphology. The collected data were analyzed using ANOVA. The findings of the research is that quality of oocytes morphology do not have significant variation to the size of cow’s ovarians. The second group produced oocytes with A 34, 25% and B 41,10% quality which was higher than the first group (22,33% and 43, 69%) and the third group (16,53 % and 32,23%). Meanwhile, for the amount of the oocytes A and B quality is higher in the second group (75, 35%). From the data, it can be conclude that the size of cow’s ovarian does not influence to the procentage of the amount of oocytes based on the quality of morphology of A and B oocytes.

Kata Kunci : sapi, ukuran ovarium, kualitas oosit.