PENGARUH SUPLEMENTASI ETHINYL ETHYL ESTRADIOL TERHADAP HISTOPATOLOGIS GINJAL TIKUS OVARIEKTOMI

NURUL ARIFAH, Dr.drh.Hartiningsih, M.P.

2014 | Skripsi | KEDOKTERAN HEWAN

Ovariektomi menurunkan produksi hormon estrogen yang mengakibatkan peningkatan ekskresi kalsium melalui ginjal. Suplemen estrogen, meningkatkan estrogen dan menurunkan ekskresi kalsium urin, namun suplemen estrogen juga menurunkan paratiroid hormon dan meningkatkan ekskresi kalsium urin. Penelitian ini bertujuan untukmengetahui gambaran histopatologis ginjal tikus ovariektomi yang mengkonsumsi suplemen ethinyl ethyl estradiol.Sepuluh tikus wistar betina umur 8 minggudibagi dalam 2 kelompok, tikus ovariektomi (OVK) dan tikus ovariektomi yang diberi suplemen ethnyl ethyl estradiol 25 g/tikus/hari secara oral (OVE), masing-masing 5 tikus dan diberi pakan standar dan air minum aquabidestilata secara ad libitum. Tikus ditempatkan dalam kandang individu dengan suhu ruang berkisar 22-25 . Tikus diadaptasikan terhadap pakan dan lingkungan selama 1 minggu, pada umur 9 minggu dilakukan operasi ovariektomi. Satu minggu pasca operasi, semua tikus diberi perlakuan selama 8 minggu. Pada akhir perlakuan, tikus dietanasi dan diambil ginjal sinister untuk pemeriksaan histopatologis dengan pengecatan HE kemudian dianalisis secara deskriptif.Hasil penelitian menunjukkan tidak ada perubahan pada gambaran histopatologis ginjal tikus ovariektomi yang diberi suplemen ethinyl ethyl estradiol. Dari hasil penelitian, disimpulkan bahwa ethinyl ethyl estradiol aman terhadap fungsi ginjal. Kata Kunci : Ethinyl ethyl estradiol, ginjal, ovariektomi

Ovariectomy decrease the production of estrogen hormone resulted in an increase in the calcium excretion through kidney. Supplement estrogen, increase estrogen and decrease calcium excretion of urine, however supplement estrogen also decrease parathyroid hormone and increase calcium excretion of urine. This research aimed to know the description of histopatologis kidney of rats ovariektomi who consumed supplement of ethinyl ethyl estradiol. Ten female wistar rats aged eight weeks were divided into 2 groups, rat ovariectomy (OVK) and mouse ovariectomy which fed supplement of ethinyl ethyl estradiol 25 g/rats/day orally (OVE). Each five rats, fed with standard woof and drinking water aquabidestilata in ad libitum. Rats placed in an individual cage with 22-25 of room temperature. Rats adapted to woof and environment for one week, at the age of nine weeks, performed ovariektomi surgery. One week after surgery, all of the mice were given treatment during eight weeks. In the end of treatment, rats were euthanized and taken the sinister kidney for histopatologi check up with painting of HE than analyzed by descriptive. The result showed that there is no change on the illustration of histopatologiskidney of rats ovariectomy who fed with supplement of ethinyl ethyl estradiol. From the result of the research, concluded that ethinyl ethyl estradiol is save against the kidney function. Keywords: Ethinyl ethyl estradiol, kidney, ovariectomy

Kata Kunci : Ethinyl ethyl estradiol, ginjal, ovariektomi