PENGARUH VARIASI PEMBERIAN FORTIFIKAN ZAT BESI DAN ZINK PADA SUSU FERMENTASI SINBIOTIK (Lactobacillus plantarum Dad-13–Fruktooligosakarida) TERHADAP KADAR HEMOGLOBIN TIKUS GALUR WISTAR

FAISHAL HANIN, Siti Helmyati, DCN., M.Kes.

2014 | Skripsi | GIZI KESEHATAN

Latar Belakang: Anemia merupakan masalah kesehatan yang prevalensinya terus meningkat. Anemia dapat menurunkan derajat kesehatan dan produktivitas kerja. Pengembangan pangan fungsional dengan fortifikasi mikro mineral berpotensi mengatasi anemia. Fortifikasi zat besi dan zink pada susu fermentasi sinbiotik diharapkan dapat meningkatkan kadar hemoglobin dan menurunkan prevalensi anemia. Tujuan: Diketahuinya pengaruh variasi pemberian fortifikan zat besi dan zink pada susu fermentasi sinbiotik (Lactobacillus plantarum Dad-13– Fruktooligosakarida) terhadap kadar hemoglobin tikus galur wistar. Metode: Penelitian eksperimental dengan pretest and posttest control group design. Tikus galur wistar sebanyak 36 ekor diadaptasi selama 1 minggu kemudian dibagi dalam 3 kelompok yaitu kelompok kontrol yang diberi susu fermentasi sinbiotik tanpa fortifikasi, kelompok perlakuan I yang diberi susu fermentasi sinbiotik yang difortifikasi NaFeEDTA dan Zn asetat, dan kelompok perlakuan II yang diberi susu fermentasi sinbiotik yang difortifikasi Fe glukonat dan Zn asetat. Serum darah diambil sebelum dan sesudah perlakuan untuk diukur kadar hemoglobinnya. Analisis data menggunakan uji statistik one way ANOVA untuk mengetahui perbedaan antar kelompok dan paired t-test untuk mengetahui signifikansi peningkatan kadar hemoglobin dalam kelompok. Hasil: Terjadi peningkatan kadar hemoglobin pada kelompok perlakuan dan kelompok kontrol. Peningkatan kadar hemoglobin ketiga kelompok tidak berbeda signifikan (p > 0,05) dan nilai peningkatannya pun tidak signifikan (p > 0,05). Peningkatan kadar hemoglobin kelompok NaFeEDTA - Zn asetat sebesar 15,39%, kelompok Fe glukonat – Zn asetat sebesar 11,05%, dan kelompok kontrol sebesar 9,01%. Kesimpulan: Variasi pemberian fortifikan zat besi dan zink pada susu fermentasi sinbiotik (Lactobacillus plantarum Dad-13–Fruktooligosakarida) tidak berpengaruh signifikan terhadap kadar hemoglobin tikus.

Background: anemia is one of health problems, in which its prevalence always increases. It can decrease health degree and work productivity. Functional food development with micro mineral fortification in food can be the solution to overcome anemia. Ferrous and zinc fortification in sinbiotic fermented milk is expected to be able to increase hemoglobin level, thus it decreases anemia prevalence. Objective: this study was carried to investigate the effect of ferrous and zinc fortification variation in sinbiotic fermented milk (Lactobacillus plantarum dad-13– Fruktooligosakarida) to hemoglobin level of wistar rats Method: this study design was true experimental with pretest and posttest control group design. Thirty six wistar rats were adapted for one week then divided into three groups. First, control group was provided with sinbiotic fermented milk without fortification. Second, NaFeEDTA – Zn acetate group was provided with sinbiotic fermented milk fortified by NaFeEDTA and Zn acetate. And third, Fe gluconate – Zn acetate group was provided by sinbiotic fermented milk fortified by Fe gluconate and Zn acetate. Blood serums of rats were taken before and after treatment then their hemoglobin level were measured. Data was analyzed statistically using one way analysis of variance (ANOVA) and paired t-test to know the increase significance of hemoglobin level in groups. Result: there was found hemoglobin level increase in the three groups. The differences of hemoglobin level increase among groups were not significant, (p > 0.05). The value of hemoglobin level increase in each group was not significant (p > 0.05). Hemoglobin level increase in NaFeEDTA – Zn acetate group was 15.39 %; Fe gluconate – Zn acetate group was 11.05 %; and control group was 9.01%. Conclusion: variation of ferrous and zinc fortification in sinbiotic fermented milk (Lactobacillus plantarum Dad-13–Fruktooligosakarida) has no significant effect to hemoglobin level of wistar rats.

Kata Kunci : Susu fermentasi sinbiotik, Fortifikasi, Kadar Hemoglobin