KECERNAAN IN VITRO MONOGASTRIK BUNGKIL INTI KELAPA SAWIT FERMENTASI MENGGUNAKAN ISOLAT : BAKTERI SELULOLITIK BELALANG KEMBARA (Lokusta migratoria L.)

BISMO ADITYO, Prof. Dr. Ir. Lies Mira Yusiati, SU.

2014 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh fermentasi dengan penambahan isolat bakteri selulolitik dari belalang kembara (Lokusta migratoria L) terhadap kecernaan in vitro bungkil inti kelapa sawit (BIKS). Bakteri selulolitik didapat dari cairan isi perut belalang yang telah dilakukan pengkayaan dan pertumbuhan. Fermentasi BIKS akan dilakukan dengan perbedaan jumlah level isolat yang digunakan sebanyak 0, 10, 20 dan 30% kemudian waktu fermentasi yaitu 21 hari. Setelah itu dianalisis komposisi kimia meliputi serat kasar (SK), protein kasar (PK), lemak kasar (LK), bahan kering (BK), dan bahan organik (BO). Kecernaan diukur dengan metode in vitro monogastrik meliputi kecernaan bahan kering (KcBK), kecernaan bahan organik (KcBO), kadar protein tercerna dan gula reduksi. Data yang diperoleh dianalisis menggunakan analisis variansi pola searah kemudian dilanjutkan dengan Duncan’s new multiple range test (DMRT). Penurunan SK dan kenaikan BETN pada hasil fermentasi berakibat naiknya kadar gula pada produk hasil in vitro. Penambahan level inokulum selulolitik sebesar 10%, 20%, dan 30% menyebabkan kenaikan kadar gula mereduksi BIKSF sebesar 12,39%, 20,33% dan 27,98% (P<0,05) dibanding dengan kontrol selain itu penambahan level inokulum sebesar 20% dan 30% menaikkan kadar protein sebesar 22,25% dan 21,74% (P<0,05) dibandingkan dengan kontrol. Penambahan level inokulum selulolitik sebesar 20 dan 30% dapat menaikan kecernaan bahan kering (KcBK) sebesar 11,89 dan 13,37% (P<0,05) serta kecernaan bahan organik (KcBO) naik sebesar 18,02 dan 22,81% (P<0,05) dibanding kontrol. Berdasarkan hasil penelitian yang telah dilakukan dapat diambil kesimpulan bahwa fermentasi bungkil inti kelapa sawit dengan penambahan bakteri selulolitik belalang kembara (Lokusta migratoria L) sebesar 20% dapat meningkatkan kecernaan nutrien secara in vitro. Kata kunci : Bungkil inti kelapa sawit fermentasi, Isolat seluloitik, Kecernaan in vitro, Belalang kembara.

This reasearch was conducted to determine the effect of fermentation with the addition of cellulolytic bacteria which had isolated from migratory grasshopper (Lokusta migratoria L) on in vitro digestibility of palm kernel cake (PKC). Cellulolytic bacteria isolated from extract digestive gut of grasshopper that had been enriched and grows. Fermentation PKC was done with different levels of the isolates by 0, 10, 20, and 30% then fermented for 21 days. Fermented PKC was analyzed chemical composition including crude fiber, crude protein, crude fat, dry matter, and organic matter. The digestibility was measured by the method of the monogastric in vitro which including dry matter digestibilty, organic matter digestibility, digested protein content (Lowry) and reducing sugar. Data were analyzed using analysis of variance test pattern in unidirectional the Duncan’s new multiple range test (DMRT). The decline of crude fiber and increased nitrogen free extract on fermented sugar levels rise resulting in a outcome product in vitro. Addition of cellulolytic inoculum levels by 10%, 20% and 30% led to increased levels of reducing sugar content in PKC at 12,39%, 20,33% and 27,98% (P<0,05) compared with controls other than the addition of inokulum by 20% and 30% increased the protein content by 22,25 and 21,74% (P<0,05) compared with controls. Addition of cellulolytic inoculum level by 20 and 30% increased the digestibility of dry matter 11,89 and 13,37% (P<0,05) and organic matter rose by 18,02 and 22,81% (P<0,05) compared to controls. Based on the research that has been done can be concluded that the fermentation of palm kernel cake with the addition of cellulolytic bateria from migratory grasshopper (Lokusta migratoria L) by 20% can increase nutrient digestibility in vitro. Key words : Palm kernel cake fermentation, Cellulolytic isolate, In vitro digestibility, Migratory grasshopper

Kata Kunci : Bungkil inti kelapa sawit fermentasi, Isolat seluloitik, Kecernaan in vitro, Belalang kembara, Palm kernel cake fermentation, Cellulolytic isolate, In vitro digestibility, Migratory grasshopper