AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK LAMUN Cymodocea sp.

ALVIKA HAYYU C P, Dr. Amir Husni, S.Pi., M.P.

2014 | Skripsi | TEKNOLOGI HASIL PERIKANAN

Telah dilakukan penelitian untuk mengetahui bagian-bagian lamun Cymodocea sp. yang berpotensi sebagai antioksidan dan uji toksisitasnya. Ekstrak daun dan batang Cymodocea sp. dibuat dengan cara maserasi menggunakan tiga macam pelarut, yaitu metanol, etil asetat, dan n-heksan. Pengujian kadar total fenol menggunakan metode Follin-Ciocalteu. Aktivitas antioksidan dilakukan dengan metode spektrofotometri menggunakan DPPH (1,1- diphenyl-2-picrylhydrazyl) sedangkan kadar toksisitas dilakukan dengan uji BSLT (Brine Shrimp Lethality Test). Hasil penelitian menunjukkan bahwa ekstrak metanol daun Cymodocea sp. mempunyai kandungan total fenol (26,73 mg GAE/gram ekstrak) paling tinggi dengan aktivitas antioksidan (IC50) sebesar 518,57 ppm. Berdasarkan nilai IC50, sampel tersebut tergolong antioksidan lemah. Hasil uji toksisitas menunjukkan semua sampel Cymodocea sp. bersifat toksik terhadap larva udang Artemia salina Leach. Ekstrak etil asetat daun Cymodocea sp. mempunyai toksisitas terbesar dengan nilai LC50 sebesar 67,14 ppm. Uji fitokimia menunjukkan bahwa ekstrak Cymodocea sp. mengandung senyawa golongan saponin, steroid, flavanoid, fenol hidrokuinon, dan terpenoid. Kata kunci : Cymodocea sp., antioksidan, DPPH, BSLT, Artemia salina

A research has been done to find out the part of seagrass Cymodocea sp. which has potential of antioxidant and to evaluate its toxicity. Leaves and stems extract of Cymodocea sp. were made by macerating with three kinds of solvent, i.e. methanol, ethyl acetate, and nhexane. The examination of rate total phenol was determined using Follin-Ciocalteu method. The laboratory experiments of antioxidant activities was done by spectrophotometrical method using DPPH (1,1-diphenyl-2-picrylhydrazyl) while the level of toxicity was done by BSLT (Brine Shrimp Lethality Test). The results showed that methanol extract of Cymodocea sp. leaves had the highest in total phenolic compounds (26.73 mg GAE/gram extract) with the antioxidant activity (IC50) of 518.57 ppm. Based on value of IC50, sample that mentioned appertained weak antioxidant. The determination of toxicity result showed that all samples of Cymodocea sp. were toxic to the shrimp larva Artemia salina Leach. The ethyl acetate extract of Cymodocea sp. leaves had the most toxicity with the LC50 of 67.14 ppm. The phytochemical test showed that Cymodocea sp. extract contained saponin, steroids, flavanoids, phenol hydroquinone, and terpenoids class. Keywords: Cymodocea sp., antioxidant, DPPH, BSLT, Artemia salina

Kata Kunci : Cymodocea sp., antioksidan, DPPH, BSLT, Artemia salina