UJI POTENSI DAYA ANTIBAKTERI SENYAWA FENANTROLIN 1 TERHADAP ISOLAT KLINIS BAKTERI SALMONELLA TYPHI SECARA IN VITRO

AZWAR HASHFI, dr. Titik Nuryastuti, M.Si, Ph.D

2014 | Skripsi | PENDIDIKAN DOKTER

Latar Belakang: Bakteri Salmonella typhi menjadi salah satu penyebab tersering infeksi saluran pencernaan di Indonesia. Praktik penggunaan antibiotik yang tidak tepat ikut mendorong munculnya masalah resistensi dalam pengobatan infeksi Salmonella typhi. Pengembangan antibiotik baru diperlukan sebagai salah satu upaya untuk mengatasi permasalahan resistensi bakteri terhadap antibiotik yang ada. Fenantrolin 1 merupakan sebuah senyawa baru yang disintesis dari senyawa 1,10- fenantrolin. Berdasarkan penelitian-penelitian terdahulu senyawa 1,10-fenantrolin memiliki potensi sebagai antibakteri. Tujuan: Untuk mengetahui potensi antibakteri senyawa baru turunan 1,10-fenantroline (fenantrolin 1) yang ditentukan dari nilai Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM) terhadap isolat klinik bakteri Salmonella typhi. Metode: Penelitian ini memiliki desain eksperimental deskriptif dan uji potensi antibakteri senyawa fenantrolin 1 terhadap bakteri Salmonella typhi dilaksanakan dengan metode Dilusi Cair Makro (Macrobroth Dilution) Hasil: Berdasarkan percobaan menunjukkan bahwa senyawa fenantrolin 1 mempunyai Kadar Hambat Minimum (KHM) sebesar 100 μg/ml dan Kadar Bunuh Minimum (KBM) sebesar 200 μg/ml terhadap isolat klinis bakteri Salmonella typhi. Kesimpulan: Isolat klinis bakteri Salmonella typhi mampu dihambat dan dibunuh pertumbuhannya di lingkungan in vitro oleh fenantrolin 1 pada konsentrasi tertentu.

Background: Salmonella typhi is one of the most common causes of gastrointestinal infections in Indonesia. Improper use of antibiotics contributes to the rise of resistance problems in the treatment of Salmonella typhi infection. The development of new antibiotics is needed as part of efforts to tackle the problem of bacterial resistance against the existing antibiotics. Phenanthroline 1 is a new compound that is synthesized from 1,10-phenanthroline compound. Based on previous studies, 1,10-phenantroline has quality as an antibacterial compound. Objective: To determine the potency of a new antibacterial compound derived from 1,10-Phenanthroline (Phenanthroline 1) against clinical isolates of Salmonella typhi by establishing the value of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Method: Descriptive experimental design is used in this study and Macrobroth Dilution method is chosen to determine the antibacterial potency of Phenanthroline 1 compound against Salmonella typhi. Result: Based on the experiments, Phenanthroline 1 has the Minimum Inhibitory concentration (MIC) at 100 μg/ml and Minimum Bactericidal Concentration (MBC) at 200 μg/ml against clinical isolates of Salmonella typhi. Conclusion: Phenanthroline 1 compound is, at a certain concentration, capable of inhibiting and killing clinical isolates of Salmonella typhi in in vitro environment.

Kata Kunci : Fenantrolin 1, Salmonella typhi, Dilusi Cair Makro, Kadar Hambat Minimum, Kadar Bunuh Minimum.