Uji Potensi Antijamur Senyawa Fenantrolin 2 terhadap Strain Candida sp Resisten Terbinafin dengan Metode Microbroth Dilution

JIHAN FADLILA GUBIANANDA, Dr. Titik Nuryastuti, M.Si

2013 | Skripsi | PENDIDIKAN DOKTER

Latar Belakang : Penyakit infeksi Candida atau disebut Candidiasis menjadi suatu masalah kesehatan masyarakat di Indonesia maupun di beberapa negara lain. Hampir 75% pada semua wanita dewasa setidaknya mengalami satu kali infeksi jamur Candida selama hidupnya. Sekitar 11% sampai 40% pasien menderita infeksi immunokompromis jamur sistemik dan 30% pasien meninggal karena infeksi Candida. Selain itu, kandidiasis merupakan infeksi oportunistik tertinggi pada ODHA. Kondisi ini juga diperparah dengan adanya masalah resistensi terhadap antijamur Candida, salah satunya adalah resistensi Terbinafin. Senyawa fenantrolin 2 merupakan suatu senyawa turunan dari Fenantrolin murni. Studi literatur menunjukkan bahwa senyawa fenantrolin mempunya aktivitas dalam melawan jamur Candida Sp. Tujuan : untuk mengetahui potensi antijamur senyawa Fenantrolin 2 terhadap Candida Sp resisten Terbinafin dengan metode Microbroth Dilution. Metodologi : Penelitian ini menggunakan rancangan penelitian deskriptif eksperimental. Subjek penelitian adalah Jamur Candida Sp resisten Terbinafin isolat klinis Laboratorium Mikrobiologi FK UGM. Penelitian ini menggunakan metode microbroth dilution pada media RPMI yang telah dicampur dengan senyawa Fenantrolin 2 dengan berbagai konsentrasi. Hasil : Senyawa Fenantrolin 2 mempunyai potensi antijamur terhadap Jamur Candida Sp resisten Terbinafin dengan kadar hambat minimumnya 3,175 μg/mL. Kesimpulan : Senyawa Fenantrolin 2 mempunyai kemampuan dalam menghambat laju pertumbuhan jamur Candida Sp resisten terbinafin.

Background: Candida infection disease or is called Candidiasis becomes a health problem in Indonesian society and also in some other countries. Almost 75% of all adult women have suffered from this kind of Candida infection at least once in their life. It is about 11% up to 40% of medical patients suffered from systemic fungi immunocompromised infection and 30% of the patients died because of the Candida infection. Beside, candidiasis is the highest opportunistic infection among ODHA (people who infected with HIV/AIDS). This condition becomes worsen with the existence of resistance problems with Candida anti-fungi, one of them is Terbinafine resistance. Fenantrolin 2 compound is a derivative compound from the pure Phenanthroline. Literature study showed that Phenanthroline compound has an activity againsts the Candida sp. fungi. Purpose: The objective of this study is to know the potential of Fenantrolin 2 anti-fungi compound towards the Candida sp. resistant Terbinafine by using Microboth Dilution method. Methodology: This study used descriptive experimental research design. Subject of the study was Candida sp. fungi resistant Terbinafin clinical isolate Microbiology Laboratory at Medical Faculty Gadjah Mada University. This study was done by using microboth dilution method in RPMI media which had been mixed with Fenantrolin 2 compound with various concentrations. Result: Fenantrolin 2 has an anti-fungi potential toward the Candida sp. resistant Terbinafine with the minimum inhibitory concentration of 3,175 μg/mL. Conclusion: Fenantrolin 2 compound has an ability in obstructing the growth of Candida sp. resistant Terbinafine.

Kata Kunci : Candida sp, Candidiasis, Terbinafin, Fenantrolin, metode Microbroth dilution