SENSITIVITAS Escherichia coli PENGHASIL EXTENDED SPECTRUM BETA LACTAMASE (ESBL) TERHADAP ANTIBIOTIK GOLONGAN PENISILIN DARI ISOLAT KLINIS KOLEKSI BAGIAN MIKROBIOLOGI FK UGM PERIODE APRIL – SEPTEMBER 2013

GLADIAN YANURISKA, dr. Nurrokhman, M.Si

2014 | Skripsi | PENDIDIKAN DOKTER

Abstrak : Penyakit infeksi merupakan salah satu masalah kesehatan terbesar baik di Indonesia maupun dunia. Salah satu bakteri yang paling banyak menyebabkan infeksi adalah E. coli. Infeksi paling sering ditimbulkan adalah diare dan infeksi saluran kencing. Dari berbagai penelitian, penggunaan antibiotik β- laktam khususnya penisilin dilaporkan resisten terhadap E. coli. Mekanisme paling umum terjadinya resistensi adalah produksi enzim β-laktamase oleh bakteri. Tujuan : Mengetahui sensitivitas Escherichia coli yang menghasilkan ESBL terhadap golongan penisilin dari isolat klinis koleksi Bagian Mikrobiologi FK UGM. Metode : Penelitian ini menggunakan metode deskriptif. Metode deteksi ESBL menggunakan Skrining menurut CLSI 2012 dan Mast Group Test® dan uji kepekaan menggunakan metode Kirby Bauer. Hasil : Dari 25 sampel uji, 7 sampel positif ESBL (28%). Hasil uji kepekaan terhadap antibiotik menunjukkan bahwa E. coli penghasil ESBL 100% resisten terhadap penisilin G dan ampisilin, 71,4% terhadap amoksisilin dan 85,7% terhadap tikarsilin. Sementara itu, E. coli non ESBL 100% resisten terhadap penisilin G, 88,5% terhadap ampisilin, 77,8% terhadap amoksisilin dan tikarsilin. Kesimpulan : Antibiotik golongan penisilin resisten terhadap E. coli penghasil ESBL.

Background : Infectious disease is one of biggest problem in Indonesia and even in the world. One of bacteria that cause a lot of infection is E. coli. Infection that caused by E. coli are diarrhea and urinary tract infection. According to various researches, using the β-lactam antibiotics especially penicillin group had been reported resistant to this bacteria. The general mechanism of resistant is ESBL production by bacteria. Objective : This study was design to determine sensitivity of ESBL producing isolates of E. coli toward penicillin from clinical specimens at Microbiology Departement in Faculty of Medicine Gadjah Mada University. Methods : This study used descriptive method. Screening Test from CLSI 2012 and Mast Group Test® were used to screening ESBL production. Kirby Bauer method was used for antibiotic susceptibility testing. Results : From 25 clinical samples, 7 from these were produced ESBL (28%). Result of susceptibility test to antibiotic penicillin group showed E. coli produced ESBL 100% resistant to penicillin G and ampicilin, 71,4% to amoxicillin and 85,7% to ticarcillin. While E. coli non ESBL 100% resistant to penicillin G, 88,5% to ampicillin, 77,8% to amoxicillin and ticarcillin. Conclusion : Penicillin group are resistant against E. coli.

Kata Kunci : β-laktamase, ESBL, Extended Spectrum Beta Lactamase, E. coli, penisilin.