Hubungan antara ekspresi Ki-67 dengan ekspresi VEGF-C pada karsinoma duktal invasif payudara

NOVI ANDRITIA, dr. Irianiwati Widodo, Sp. PA (K)

2014 | Skripsi | PENDIDIKAN DOKTER

Latar Belakang: Metastasis karsinoma payudara secara limfogen yang dimulai dengan proses limfangiogenesis. Ki-67 dan Vascular Endothelial Growth Factor (VEGF)-C berperan penting dalam limfangiogenesis. Hubungan ekspresi Ki-67 dan VEGF-C pada karsinoma payudara belum jelas. Tujuan: Mengetahui hubungan antara ekspresi Ki-67 dengan ekspresi VEGF-C pada karsinoma duktal invasif. Metode: 36 spesimen blok parafin karsinoma duktal invasif payudara dicat immunohistokimia dengan antibodi monoklonal dengan metode Streptavidin-Biotin, dengan antibodi anti-Ki-67 dan anti-VEGF-C. Ekspresi Ki-67 dihitung dari jumlah sel karsinoma payudara yang intinya tercat positif. Ekspresi VEGF-C dihitung dari jumlah sel sitoplama karsinoma payudara yang sitoplasmanya tercat positif. Hubungan antara masingmasing variabel dianalisis dengan metode Chi Square. Hasil: Tidak didapatkan hubungan yang signifikan antara ekspresi Ki-67 dengan ekspresi VEGF-C pada karsinoma duktal invasif payudara p= 0,790 (p<0,05). Kesimpulan: Pada penelitian ini ekspresi Ki-67 tidak berhubungan dengan ekspresi VEGF-C pada karsinoma duktal invasif payudara.

Background: Metastasis of breast carcinoma commonly through the lymphatic vessels which is initiated by lymphangiogenesis. Ki-67 and Vascular Endothelial Growth Factor (VEGF)-C have important role in lymphangiogenesis, but their correlation is still unclear. Aim : To study the correlation between Ki-67 and VEGF-C expression in ductal invasive breast carcinoma. Method: 36 paraffin embedded tissue of invasive ductal carcinoma of the breast were stained immunohistocemically using Streptavidin-Biotin method with monoclonal antibody anti Ki-67 and anti VEGF-C. Ki-67 expression was evaluated by the percentage of breast carcinoma test nuclear expressed Ki-67. VEGF-C expression was evaluated by the percentage of breast carcinoma test cytoplasm expressed VEGF-C. The relationship between each variables were analyzed by chi square method. Result: Statistical analysis showed there was no significant correlation between Ki-67 and VEGF-C expression in invasive ductal carcinoma of the breast p= 0,790 (p<0,05). Conclusion: In this study, Ki-67 expression is not correlated with the VEGF-C expression in Invasive Ductal Carcinoma of the Breast

Kata Kunci : Limfangiogenesis, Ki-67, VEGF-C, Karsinoma Duktal Invasif Payudara.