HUBUNGAN ANTARA TUMOR ASSOCIATED MACROPHAGES (TAMs) DENGAN EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR-C (VEGF-C) PADA SEL KARSINOMA PAYUDARA

RIZKA IBONITA, dr. Irianiwati, Sp.PA(K).

2014 | Skripsi | PENDIDIKAN DOKTER

Latar Belakang: VEGF-C merupakan sitokin penting pada proses limfangiogenesis karsinoma payudara. VEGF-C dihasilkan oleh sel tumor dan sel radang terutama TAMs, dan setelah berikatan dengan VEGFR-3 akan memacu proliferasi, diferensiasi, migrasi, dan maturasi sel endotel limfatika. Hubungan antara jumlah TAMs dengan ekspresi VEGF-C pada karsinoma payudara belum jelas. Tujuan: Mempelajari hubungan antara jumlah TAMs peritumor dengan ekspresi VEGF-C pada sel karsinoma payudara. Metode: Empat puluh satu blok parafin jaringan karsinoma payudara dicat dengan antibodi monoklonal anti-VEGF dan anti-CD68 dengan teknik Streptavidinbiotin kompleks dengan kromogen DAB dan pulasan balik Hematoksilin Mayer yang mengekspresi CD68. Jumlah TAMs dihitung dari banyaknya makrofag. Ditentukan nilai cutoff TAMs dan dikelompokkan menjadi tinggi dan rendah. Ekspresi VEGF-C dinilai dari persentase sel tumor yang sitoplasmanya mengekspresikan VEGF-C. Ekspresi VEGF-C dikelompokkan menjadi rendah: negatif dan positif ≤10%, tinggi: positif >10%. Hubungan jumlah TAMs dengan ekspresi VEGF-C dianalisis dengan tes chi-square. Hasil: Nilai cut-off TAMs adalah 49. Jumlah sampel TAMs tinggi : 53,7%, rendah : 46,3%. Jumlah sampel dengan ekspresi VEGF-C <10% : 12,2%, >10% : 87,8%. Tidak didapatkan hubungan yang signifikan antara jumlah TAMs dengan ekspresi VEGF-C pada sel karsinoma payudara (p=0,762). Kesimpulan: Pada penelitian ini, TAMs tidak berperan dalam limfangiogenesis yang diinduksi oleh VEGF-C pada sel karsinoma payudara.

Background: VEGF-C is an important cytokine in the lymphangiogenesis process in breast carcinoma. VEGF-C is produced by tumor cells and inflammatory cells, especially TAMs, and after binding with VEGFR-3 it can stimulate proliferation, differentiation, migration, and lymphatic endothelial cell maturation. The relationship between the number of TAMs with VEGF-C expression in breast carcinoma is unclear. Objectives: To study the relationship between the number of peritumoral TAMs with VEGF-C expression in breast carcinoma cells. Methods: Forty one breast carcinoma tissue paraffin blocks were stained with anti-VEGF monoclonal antibody and anti-CD68 with Streptavidin-biotin complex technique with DAB chromogen and Hematoxylin Mayer that expressed CD68 to counterstain. Number of TAMs was counted from the number of macrophages. Cut-off value of TAMs were determined and grouped into high and low. Expression of VEGF-C were assessed on the percentage of tumor cells, that its cytoplasm is expressing VEGF-C. Expression of VEGF-C were grouped into low: negative and positive ≤10%, high: positive >10%. Relationship between the number of TAMs with VEGF-C expression was analyzed by chi-square test. Results: TAMs cut-off value is 49. The number of samples TAMs high: 53.7%, low: 46.3%. The number of samples with expression of VEGF-C <10%: 12.2%, >10%: 87.8%. There was no significant relationship between the number of TAMS with VEGF-C expression in breast carcinoma cells (p = 0.762). Conclusion: In this study, TAMs have not play a role of VEGF-C induced lymphangiogenesis in breast carcinoma cells.

Kata Kunci : Karsinoma Payudara, Tumor Associated Macrophages (TAMs), Vascular Endothelial Growth Factor- C (VEGF-C), CD68.