PENGARUH BERBAGAI KONSENTRASI LARUTAN IRIGASI SODIUM HIPOKLORIT KOMBINASI OMEPRAZOLE 8,5% SEBAGAI ANTIBAKTERI Enterococcus faecalis

MARIA SANTINIARATRI, drg. Wigyo Hadriyanto, M.S., Sp.KG(K)

2013 | Tesis | S2 Ilmu Konservasi Gigi

Omeprazole merupakan salah satu proton-pump inhibitor yang apabila dikombinasikan dengan sodium hipoklorit (NaOCl) terbukti mempunyai daya antibakteri Enterococcus faecalis yang tinggi. Eradikasi bakteri Enterococcus faecalis sangat penting untuk mendukung tercapainya keberhasilan perawatan saluran akar. Tujuan penelitian ini adalah untuk mengetahui pengaruh berbagai konsentrasi NaOCl kombinasi Omeprazole 8,5% sebagai antibakteri Enterococcus faecalis. Suspensi Enterococcus faecalis dibuat dengan konsentrasi 108 CFU/ml kemudian dibiakkan pada media Mueller Hinton Agar (MHA). Cawan petri berisi media MHA yang digunakan dalam penelitian ini sebanyak 7 buah, masingmasing dibuat 6 sumuran dengan diameter 6 mm dan kedalaman 3 mm. Tiap-tiap cawan petri diisi dengan masing-masing kelompok perlakuan sebanyak 5 sumuran dan 1 sumuran sisanya diisi dengan akuades sebagai kontrol negatif, volume larutan yang diteteskan 50 μl. Tujuh kelompok perlakuan tersebut adalah NaOCl 0,5%, NaOCl 2,5%, NaOCl 5,25%, NaOCl 0,5% kombinasi Omeprazole 8,5%, NaOCl 2,5% kombinasi Omeprazole 8,5%, NaOCl 5,25% kombinasi Omeprazole 8,5%, dan Omeprazole 8,5% sendiri sebagai kontrol positif. Media tersebut kemudian diinkubasi selama 24 jam pada suhu 37°C. Zona hambatan yang terbentuk diukur menggunakan jangka sorong dengan ketelitian 0,02 mm. Data dianalisis dengan uji Anava satu jalur dan dilanjutkan dengan uji LSD. Hasil uji Anava satu jalur yang dilanjutkan uji LSD menunjukkan terdapat perbedaan yang bermakna (p < 0,05). Pada penelitian ini dapat disimpulkan bahwa penambahan Omeprazole 8,5% meningkatkan daya antibakteri NaOCl terhadap Enterococcus faecalis.

Omeprazole is one of proton-pump inhibitor which when combined with sodium hypochlorite (NaOCl) inherits a superior antibacterial efficacy against Enterococcus faecalis. Eradication of Enterococcus faecalis bacteria is important to achieve a successful root canal treatment. The objective of this study is to figure out the effectiveness of various concentrations of NaOCl combined with 8.5% Omeprazole as an antibacterial efficacy against Enterococcus faecalis. Suspension of Enterococcus faecalis was made in 108 CFU/ml concentration and then inoculated in Mueller Hinton Agar (MHA) medium. There were 7 petri dishes containing MHA medium used in this study, each of dish was implemented by six pits with 6 mm diameter and 3 mm depth. Each of petri dish was filled with each treatment group in 5 pits and 1 other pit was filled with aquades as negative control, solution volume drops was applied by 50 μl. Seven examined treatment groups were 0.5% NaOCl, 2.5% NaOCl, 5.25% NaOCl, 0.5% NaOCl combined with 8.5% Omeprazole, 2.5% NaOCl combined with 8.5% Omeprazole, 5.25% NaOCl combined with 8.5% Omeprazole, and 8.5% Omeprazole itself as positive control. The mediums above were incubated for 24 hours at 37°C. Inhibition zone were measured by sliding caliper in accuracy of 0.02 mm. Data were analyzed by one way Anava and followed by LSD test. Test results generated by one way Anava and followed by LSD test showed that there were significant effects occurred (p < 0.05). This study concluded that the addition of 8.5% Omeprazole increased the antibacterial efficacy of NaOCl against Enterococcus faecalis.

Kata Kunci : Sodium hipoklorit, Omeprazole, daya antibakteri, Enterococcus faecalis; Sodium hypochlorite, Omeprazole, antibacterial efficacy, Enterococcus faecalis