Pengaruh asap berbagai jenis rokok terhadap peroksidasi lipid plasma tikus putih (Rattus norvegicus, L)

YUNINGTYASWARI, Prof.Dr. H.M. Ismadi

2001 | Tesis | S2 Ilmu Kedokteran Dasar

Kesehatan tubuh dipengaruhi oleh berbagai fbktor, termasuk di mtaranya adalsh nutrisi, lingkungan, kebiasean hidup dan keseimbangan antara radikai bebas dengm &em antioksidan ddam tub&. Sdah satu kebiasnan yang dapat merugikan keshtan, antam lain adalah merolrdL Penelitiau 8cc8f8 in vitm memmjukkan bahwa asap rokok dapat medusak kesetimbangan antsra radikal bebas b g a n sistem mtioksidan tub& sehingga dapat meaimbulkaa kerusakan oksidatif jarbgan. K h a n oksidatifjariqpn hi mtara lain dapat dicenminkan dengan peroksidasi lipid. Mash terjadi kontrovgsi hasil-had penelitian men+ pgaruh pendedahan d a m asap rokok secafa in vivo. Di pasatan tadapat berbagai jenis rokok, misalnya rokok Wek, mild, putrh, filter dan non Glter. Pa&tian ini bertujuan manbuktikan bahwa asap mkok yang digunakm llntuk pendedahan hewan mba mampu * perdzsidasi lipid plasma d q ma bandingkan War MDA plasma bebaapa kelmpok hewen ooba yang &lab didedahkan secara periodik dagm asap satu jeaiS rokok tertentu sesuai dengan kdompolcnya; menennuksn jds rokok yang asapnya mempunyai pen%aruh jxding ringan tahadap peroksidasi lipid di antara bebempajenis rokok Penelitian ini meaggunakan 25 &or tikm put& (Rums nomgim) galur Spgagut Dowlqr jantan benmrur 3 bulan yang didistn'busikas ke dalam 5 kelompok, dcngan setiap kdompdc sebsmyak 5 &or. Ke lima kelcnnpok t&ut tedri dari 1 kelompok kmtrol tanpa didedahkan dengan asap rokok dm 4 kelompok perlakuan. Setiap kelmpok paial

Good body health is M u d by mauy factors, such as nutrition, environmeat, habits, and a balance between the presence of fiee radicals and antioxidant system in the body. Habits could damage health for example smoking. radicals and antioxibt system, so that it muid cause oxidative damage of the tissues. Oxidative damage of tissues among othgs could be refleaed by lipid eaamtered. There were many kinds of cigarette in the market, such as kretek, mild, filter and ncm-film cigm#tes. The aim of this experiments were to prove that cigm%e’s smoke which was used to expose test animals could influence plasana lipid peroxidation; levels of pllrsma lipid peroxidation amid be reflected * d&y& (MDA). used 25 white rads (Rattus norvegicus,L) from strain by the plasma Malandral Sprague Dowley kind male 3-mont.h age which was distributed in 5 group, with 5 meanbeas in each group. The five groups amsimd of 1 control group without being cxposad to &aretk smoke, 4 treatment pups which WBS exposed to 1 brand of cigar- smoke, during 30 minutes evexy morning amd afternoon fw 35 days for each group. The kind of agarems, Weh were used for the ~~posure, were respactiveiy kretek, filter kretck, miid and white cigumte, me brand for the treatment of 1 group. Tbe results of these experbats showed, that periodicaly exposure of test animal to cigarette smoke i n d pla sma lipid peroxidation significantly (p < 0,OS). Thac was sigaificant difference (p < 0,05) on severity of lipid perOxi&ticm that emerged from smoke cigarettes smoke used for the exposure the test snimals. Smke of white cigarette showed the lightest effect on plasma lipid peroxidation complved to the other brands of the ci-e used for the exposure oftestanima

Kata Kunci : Kesehatan, Oksidasi Lipid Plasma, Asap Rokok, cigarette smoke, h e radid, lipid peroxidation.