ANALISIS DENSITAS DAN DIVERSITAS BAKTERI PADA PERMUKAAN MIKROFON STUDIO MUSIK DI KOTA YOGYAKARTA, DAERAH ISTIMEWA YOGYAKARTA

AYU MEILYAN HARIANIH, Prof. Drs. Langkah Sembiring, M.Sc., Ph.D.

2013 | Skripsi | BIOLOGI

Berdasarkan fakta bahwa fasilitas umum dapat menjadi perantara penularan penyakit maka dilakukan penelitian mengenai densitas dan diversitas bakteri pada permukaan mikrofon di tiga studio musik Yogyakarta. Pengambilan sampel dilakukan pada 3 tempat studio musik yang berbeda di Yogyakarta dengan menggunakan metode swab pada permukaan bagian kepala dan tangkai mikrofon, kemudian dilakukan penentuan densitas bakteri dengan menggunakan metode Standard Plate Count yang dinyatakan dalam cfu/cm2. Selanjutnya dilakukan isolasi dan purifikasi untuk tiap-tiap koloni yang tumbuh sampai diperoleh kultur murni (isolat). Karakterisasi meliputi morfologi koloni dan sel, sifat pengecatan, serta uji biokimiawi dan identifikasi dengan metode profile matching (generic assignment). Hasil penelitian menunjukkan bahwa rerata densitas pada permukaan mikrofon bagian kepala yaitu 3,12 x 108 cfu/cm2 dan 0,34 x 108 cfu/cm2 pada bagian tangkai mikrofon. Hasil isolasi menunjukkan bahwa diperoleh 11 kultur murni yaitu G1S1, G1S2, G1S3, G1S4, G1S5, G2S1, G2S2, G2S3, G2S4, G3S1, dan G3S2. Berdasarkan hasil karakterisasi dan identifikasi terdapat 7 genera yang berbeda meliputi anggota genus Lactobacillus (G2S1, G2S2, G2S4, G3S2), Listeria (G3S1), Staphylococcus (G1S5), Legionella (G1S4), Dermabacter (G1S3), Corynebacterium (G1S2), dan Bacillus (G1S1, G2S2). Dengan demikian dapat disimpulkan bahwa rerata densitas pada permukaan mikrofon baik pada bagian kepala maupun tangkai di studio musik cukup tinggi, sehingga berpotensi sebagai agensia penular mengingat bahwa ada strain yang diduga bersifat patogenik yaitu Listeria, Staphylococcus, Legionella, dan Corynebacterium.

Based on the fact that public facility could mediate the dispersion of contagious disease, the study on the density and diversity of bacteria on microfon surface was carried out. The sampling conducted at 3 different places music studio in Yogyakarta using the swab method on the surface of the head and stick of the microphone. The density of bacteria was determination by using the Standard Plate Count expressed in cfu/cm2. Isolation and purification of each colony. It’s different colony was isolated and purificated by streak plate and spread plate method. Characterization included colony and cell morphology, staining and biochemical tests and identification using by the method of profile matching (generic assignment). The results of the study showed that bacterial density was 3.12x108 cfu/cm2 on the surface of the microphone head and 0.34x108 cfu/cm2 on the stick of the microphone. Based on characterization of colonial and cell morphology, staining, and biochemical test, the 11 isolates were identified to be member of seven genera. Four isolates (G2S1, G2S3, G2S4, G3S2) to be member of the genus Lactobacillus, Listeria (G3S1) Staphylococcus (G1S5), Legionella (G1S4), Dermabacter (G1S3), Corynebacterium (G1S2), and Bacillus (G1S1, G2S2). It can be concluded that the mean density on the surface of the microphone either on the head or stick at the 3 music studios in Yogyakarta was relatively high. Thefore it could be that as public facilities the music studio could act as a media dispersion of bacteria let alone 4 genera among which could contain pathogenic strains, those are Listeria, Staphylococcus, Legionella, dan Corynebacterium.

Kata Kunci : bakteri, mikrofon, densitas, keanekaragaman, studi musik