DIVERSITAS DAN POTENSI OKSIDASI METANA KONSORSIUM BAKTERI DENITRIFYING- METHANOTROPHIC PADA TANAH PERTANIAN PADI

ARDIAN PRASETYA, Prof. Langkah Sembiring, M.Sc., Ph.D.

2013 | Tesis | S2 Biologi

Penelitian ini bertujuan untuk mengetahui pengaruh kadar salinitas tanah pertanian padi serta jenis pupuk terhadap diversitas dan aktivitas oksidasi metana oleh konsorsium bakteri denitrifying-methanotrophic. Diversitas konsorsium bakteri denitrifying-methanotrophic pada tanah pertanian padi yang diberi perlakuan dianalisis secara unculture dependent dengan menggunakan PCR-DGGE dan sequencing gen marker 16S rRNA sebagai dasar rekonstruksi phylogeny tree serta secara culture dependent dengan teknik isolasi selektif pada medium nitrate mineral salts (NMS). Jenis media tanam, pupuk, dan kadar salinitas berpengaruh terhadap potensi produksi dan oksidasi metana. Media tanam kompos dan pupuk NPK memiliki pengaruh stimulasi potensi oksidasi metana konsorsium bakteri denitrifyingmethanotrophic. Kadar salinitas memiliki pengaruh inhibisi terhadap potensi oksidasi metana konsorsium bakteri denitrifying-methanotrophic. Kadar salinitas memiliki pengaruh yang lebih rendah terhadap diversitas konsorsium bakteri denitrifyingmethanotrophic dibandingkan dengan jenis pupuk dan media tanam.

The objective of this research was to study the effect of soil salinity and type of fertilizer toward diversity and methane-oxidation potential of denitrifyingmethanotrophic consortia. Diversity of denitrifying-methanotrophic consortia was analyzed by uncultured-dependent method by PCR-DGGE and 16S rRNA sequencing, also by culture-dependent method by selective isolation at nitrate mineral salts (NMS) medium. Type of plant growing medium, type of fertilizer, and degree of soil salinity had impact on methane-oxidation and methane-production potential. The compost, as plant growing medium, and NPK, as fertilizer, had stimulating effect on methane-oxidation potential of denitrifying-methanotrophic consortia, whereas soil salinity had inhibiting effect on methane-oxidation potential of denitrifyingmethanotrophic consortia. Degree of salinity had lower impact to diversity of denitrifying-methanotrophic consortia than which of fertilizer and plant growing type.

Kata Kunci : konsorsium bakteri denitrifying-methanotrophic, diversitas, oksidasi metana, salinitas, pupuk