POTENSI YOGOAT (YOGURT SUSU KAMBING) TERHADAP PERBAIKAN KADAR HEMOGLOBIN, JUMLAH LEKOSIT, DAN JUMLAH LIMFOSIT TIKUS WISTAR MALNUTRISI

SUNRISE DEWANTARA SATRIA, Dr. rer nat. dr. BJ Istiti Kandarinah

2013 | Skripsi | PENDIDIKAN DOKTER

diderita anak di Indonesia. Kondisi malnutrisi mengakibatkan penurunan kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit. Pemberian YoGoat(yogurt susu kambing) dengan kandungan nutrisi dan probiotik diharapkan dapat memperbaiki kadar Hemoglobin, jumlah lekosit, jumlah limfosit. Tujuan Penelitian: mengetahui pemberian YoGoat perbedaan kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit pada tikus Wistar malnutrisi. Metode Penelitian: Empat belas ekor tikus putih jantan galur wistar umur rata-rata 3 minggu dan berat antara 35-50 gram diberikan diet pakan 50% dari pakan normal selama 21 hari untuk mencapai kondisi malnutrisi tingkat sedang (penurunan 25-39% berat badan dari berat normal). Data berat badan diambil setiap 3 hari sekali dan sisa pakan setiap hari. Setelah kondisi malnutrisi tingkat sedang tercapai setiap subyek dilakukan pengukuran kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit (pretest). Lalu subyek dibagi secara acak menjadi 2 kelompok, tiap kelompok 7 ekor. Kelompok I diberi diet pakan 50% dan kelompok II diberi diet pakan 50% dan YoGoat 2 mL/100 gram BB/hari selama 7 hari. Setelah 7 hari setiap subyek dilakukan pengukuran kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit (posttest). Efek YoGoat dinilai dengan membandingkan delta kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit pada kedua kelompok dangan menggunakan uji Mann Whitney. Hasil Penelitian: Pada kelompok malnutrisi tanpa intervensi pemberian YoGoat, berpengaruh secara signifikan terhadap penurunan kadar Hemoglobin (p<0.05) sedangkan jumlah lekosit dan jumlah limfosit tidak terdapat penurunan yang signifikan (p<0.05). Pada kelompok malnutrisi dengan intervensi pemberian YoGoat tidak terdapat perbedaan kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit yang signifikan (p<0.05). Hasil selisih kadar Hemoglobin, jumlah lekosit, dan jumlah limfosit antara kelompok malnutrisi dengan intervensi YoGoat dengan kelompok tanpa intervensi YoGoat menunjukkan hasil tidak signifikan (p<0.05). Kesimpulan: Pemberian YoGoat pada tikus Wistar malnutrisi tingkat sedang tidak mempengaruhi kadar Hemoglobin, jumlah lekosit dan jumlah limfosit darah hewan coba.

BACKGROUND: Malnutrition is a problem that affects many children in Indonesia. This Condition may result in decreased hemoglobin level, total leukocyte, and lymphocytes count. Giving YoGoat (goat milk yogurt) with nutrition and probiotics are expected to improve hemoglobin levels, total leukocyte, lymphocyte count. Objective: to determine the effects of YoGoat intake in hemoglobin level, total leukocytes, and lymphocytes count in malnourished Wistar rats. Methods: Fourteen male white rats of Wistar strain with age average of 3 weeks and weigh between 35-50 grams gave a diet 50 % of the normal feed for 21 days to reach moderate state malnutrition (weight reduction of 25-39 % from normal weight). Weight data taken every 3 days and the remains of the feed taken every day. Once the conditions are met malnutrition rate of each subject was measured hemoglobin levels, total leukocyte, and lymphocytes count (pretest). Then the subjects were randomly divided into 2 groups, each group of 7 animals. The first group ware given diets 50% cuts of total intake food and group II were 50% cuts of total intake food and feed YoGoat 2 ml/100g body weight/day for 7 days. After 7 days of each subject was measured the hemoglobin level, total leukocyte, lymphocytes count (posttest). The effect of YoGoat assessed by comparing of delta hemoglobin level, total leukocyte, lymphocytes count in both groups using the Mann Whitney test. Results: The malnourished group without YoGoat intervention had an effect on decreased hemoglobin level significantly (p<0.05), while total leukocyte and lymphocyte counts, hadn’t significant decrease (p<0.05). The malnourished group with YoGoat intervention hadn’t significant difference in hemoglobin level, total leukocyte, and lymphocytes count (p<0.05). The Hemoglobin level, Total leukocytes, and lymphocytes count between malnourished group without YoGoat intervention and malnourished group with YoGoat intervention showed no significant results (p<0.05). Conclusion: YoGoat does not give significant effects in hemoglobin levels, total leukocyte, and lymphocyte counts on Malnourished Wistar rats.

Kata Kunci : Malnutrisi, YoGoat (yogurt susu kambing), kadar Hemoglobin, jumlah lekosit, jumlah limfosit.