PENGARUH APLIKASI TOPIKAL CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE (CPP-ACP) TERHADAP PERTUMBUHAN Streptococcus alpha DAN AKUMULASI PLAK GIGI
Maria Andrini, Dr.drg. Indah Titien S, SU, Sp.KGA (K).
2013 | Tesis | S2 Ilmu Kesehatan Gigi AnakPlak merupakan salah satu penyebab timbulnya penyakit gigi dan mulut pada anak, sehingga perlu dikontrol baik secara mekanis maupun kimiawi. Salah satu kontrol plak secara kimiawi adalah aplikasi topikal yang mengandung kasein. Casein Phospopeptide-Amorphous Calcium Phosphate (CPP-ACP) merupakan bahan yang mampu menghambat perlekatan bakteri pada permukaan gigi, sehingga mampu menghambat pertumbuhan bakteri dan akumulasi plak. Tujuan penelitian ini untuk mengetahui pengaruh aplikasi topikal CPP-ACP terhadap pertumbuhan S.alpha dan akumulasi plak gigi. Subyek penelitian terdiri 22 anak, usia 3-6 tahun. Aplikasi topikal CPPACP dilakukan sekali sehari, sebelum tidur malam, selama 28 hari. Data pertumbuhan S.alpha diperoleh dengan mengusap cotton bud steril pada permukaan bukal gigi geligi rahang atas dan bawah, kemudian diinkubasi selama 24 jam. Data skor plak diperoleh dengan pengukuran skor plak metode PHPM (Podshadley dan Haley, 1968, modifikasi Amith dkk., 2007). Pengukuran jumlah koloni S.alpha dan skor plak dilakukan sebelum dan setelah aplikasi hari ke-7, 14 dan 28. Analisa data digunakan Repetead ANOVA dilanjutkan Pair Wise Comparison (LSD). Hasil penelitian menunjukkan pertumbuhan S.alpha sebelum aplikasi topikal CPP-ACP tertinggi (549,45.104 ± 146,608.104 CFU/ml) dan terendah setelah 28 hari (142,18.104 ± 26,005.104 CFU/ml). Skor plak sebelum aplikasi tertinggi (4.1182 ± 1.01589) dan terendah setelah 28 hari aplikasi topikal CPPACP (2.2682 ± .45918). Uji Repetead ANOVA dilanjutkan Pair Wise Comparison (LSD) menunjukkan perbedaan bermakna antar aplikasi (p<0.05). Disimpulkan, semakin sering aplikasi topikal CPP-ACP, semakin rendah pertumbuhan S.alpha dan akumulasi plak.
Plaque is one of the causes of oral disease in children. This plaque needs to be controlled mechanically and chemically. One of chemical plaque control is a topical application that contains casein. Casein-Amorphous Calcium Phosphate Phospopeptide (CPP-ACP) is a substance can inhibit the attachment of bacteria plaque on the teeth. We expect that it will inhibit the growth of alpha- Streptococcus so that plaque acumulation also decrease. The aim of this study is to investigate the influence of topical application of CPP-ACP to the growth of alpha-Streptococcus and dental plaque acumulation. The study subjects consisted of 22 children, aged 3-6 years. Topical application of CPP-ACP was once a day, before bedtime, in 28 days. Data the growth of alpha-Streptococcus was performed by swabbing plaque in the buccal surfaces of maxillary and mandibulary teeth with sterilized cotton bud then inoculated for 24 hours. The plaque score were measured by PHPM methods (Podshadley and Haley, 1968, modification Amith et al., 2007). Measuring the growth of alpha-Streptococcus and the plaque score taken before, after 7, 14, 28 days the application. Data were analyzed by Repeated ANOVA resumed Pair Wise Comparison (LSD). The result showed that the growth of alpha-Streptococcus before topical application of CPP-ACP was highest (549,45.104±146,608.104 CFU/ml), after 28 days application was lowest (142,18.104±26,005.104 CFU/ml). The plaque score before topical application was highest (4.1182 ± 1.01589), after 28 days application was lowest (2.2682 ± .45918). The result of Repeated ANOVA resumed Pair Wise Comparison (LSD) showed that those were significant differences (p<0.05). In conclusion, the more frequent topical application of CPPACP, the lowest growth of alpha-Streptococcus and plaque acumulation.
Kata Kunci : CPP-ACP, pertumbuhan S.alpha, akumulasi plak
