TRANSOVARIAL TRANSMISSION OF DENGUE VIRUS DETECTED BY IMMUNOHISTOCHEMICAL ASSAY AND REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION IN PLOSOKUNING, SLEMAN, YOGYAKARTA
SYAZWAN BIN MOHD SUBRI, Dr. drh. Sitti Rahmah Umniyati, SU
2013 | Skripsi | PENDIDIKAN DOKTER-
Background: Dengue Hemorrhagic Fever is a disease spread by Aedes mosquitoes. Dengue Hemorrhagic Fever is caused by four serotypes of dengue viruses. There are 2 ways of dengue virus transmission, horizontal transmission and vertical transmission. Since the most common way of dengue control is by the control of adult mosquitoes, vertical transmission of dengue virus is not addressed. Aim: The first aim is to investigate transovarial transmission of dengue virus and serotypes in Plosokuning, Minomartani village RT24 RW10, Sleman, Yogyakarta. The second aim is to study the entomological indices of Minomartani village. Methods: This is an analytical observational study. Purposive sampling of larva samples is collected from Minomartani village and colonized. The mosquitoes are studied for dengue virus. The dengue virus is detected by RT-PCR and immunohistochemical assay using monoclonal antibody DSSE10. Results: The results of the study shows transovarial transmission of dengue virus is detected in Plosokuning with dengue serotypes dengue-1 and dengue-3. Entomological indices show House Index= 43.75%, Container Index= 43.18%, Breteau Index= 59.38%, Pupae Index= 125, and Transovarial Transmission Index= 28.57%. Conclusion: It is confirmed that there is transovarial transmission of dengue-1 and dengue-3 virus among Ae. aegypti mosquitoes in Plosokuning Minomartani village, and the entomological indices support high transmission of dengue virus in those area.
Kata Kunci : Aedes aegypti, transovarial, RT-PCR, immunocytochemical
