STUDI TRANSPOR KREATININ MENGGUNAKAN MEMBRAN KOMPLEKS POLIELEKTROLIT (PEC) KITOSAN-PEKTIN

NI PUTU SRI AYUNI, Dr. Dwi Siswanta, M.Eng.

2013 | Tesis | S2 Ilmu Kimia

Penelitian tentang studi transpor kreatinin dengan menggunakan membran PEC kitosan-pektin telah dilakukan. Membran disintesis dengan variasi komposisi kitosan dan pektin (70:30), (80:20), (90:10), kemudian digunakan untuk transpor kreatinin dengan konsentrasi 70, 100, dan 130 ppm. Membran dengan hasil difusi kreatinin optimum digunakan untuk mensintesis membran PEC kitosan-pektin MIP (Molecularly Imprinted Polymer). Membran kitosan-pektin non MIP dan MIP juga digunakan untuk transpor kompetisi kreatinin, urea dan vitamin B12. Membran PEC Kitosan-Pektin disintesis dan dikarakterisasi dengan spektrofotometer IR, pengukuran kuat tarik dan regangan, analisis SEM. Kreatinin dan campuran 3 kompetisi (kreatinin, urea, dan vitamin B12) yang dilewatkan pada membran kemudian dianalisis jumlah kreatinin yang terdifusi dan nilai fluks pada membran kitosan pektin non-MIP maupun MIP. Hasil karakterisasi membran PEC kitosan-pektin dengan spektrofotometer IR menunjukkan adanya interaksi ionik antara gugus karboksil dari pektin dan gugus amino dari kitosan pada bilangan gelombang 1604 cm-1. Perbandingan komposisi membran non MIP yang optimum adalah 70:30. Membran PEC kitosan-pektin 70:30 dengan konsentrasi awal kreatinin berturut-turut 70; 100; 130 ppm menghasilkan kreatinin yang terdifusi 24,29; 24,63; 25,24% dan nilai fluks 3,87×10-4; 6,34×10-4; 6,53×10-4 g/m2s. Jumlah molekul kreatinin yang terdifusi menggunakan membran MIP 26,80; 27,53; 28,59% dan nilai fluks 5,88×10-4, 6,56×10-4, 7,00×10-4 g/m2s. Hasil transpor kompetisi antara kreatinin, urea dan vitamin B12 menggunakan membran PEC kitosan-pektin non MIP menghasilkan urea yang terdifusi lebih tinggi sedangkan membran MIP kreatinin yang terdifusi lebih tinggi.

A study of creatinine transport using chitosan-pectin polyelectrolyte complex (PEC) membranes had been conducted. Membranes were synthesized with variations of the chitosan-pectin PEC membranes composition of (70:30), (80:20), (90:10) and later used for creatinine transport with concentrations of 70, 100, dan 130 ppm. Membranes with the optimum creatinine diffusion result were used to synthesize Molecularly Imprinted Polymer (MIP). The MIP and non-MIP chitosan-pectin membranes were also used for the competition of creatinine, urea and vitamin B12 transport. Chitosan-pectin PEC membranes were synthesised and characterised using the infrared (IR) spectrophotometer, measurement of tensile strength and strain, scanning electron microscopy (SEM) analysis. Then, Creatinine and solution of the three competitions (creatinine, urea and vitamin B12) that has been passed through the membranes were analysed for the diffused creatinine and flux values in the MIP or non-MIP chitosan-pectin membranes. The results of chitosan-pectin PEC membrane characterization using IR spectrophotometer indicated the presence of ionic interactions between the carboxyl groups of pectin and amino groups of chitosan at wave number 1604 cm-1. The best result of non-MIP membrane transport composition ratio was 70:30. The initial concentrations of creatinine were 70, 100, and 130 ppm, resulting diffusions of creatinine of 24.29, 24.63, and 25.24% with flux values of 3.87×10-4, 6.34×10-4 and 6.53×10-4 g/m2s, respectively. The results of diffusion of creatinine using MIP membrane were 26.80, 27.53 and 28.59% with flux values of 5.88 ×10-4, 6.56×10-4 and 7.00×10-4 g/m2s. Result of the competition transport between creatinine, urea and vitamin B12 using non-MIP chitosan-pectin PEC membrane produced higher diffused urea. Meanwhile, higher diffused creatinine was produced in membranes with MIP.

Kata Kunci : membran, polielektrolit, kitosan, pektin, kreatinin