PENGEMBANGAN PLESTER NATRIUM FLUORIDA DENGAN ENHANCER DALAM KEDOKTERAN GIGI : PROSPEK PENGHANTARAN TRANSDERMAL SECARA IN VITRO DAN IN VIVO

drg. Diyah Fatmasari, MDSc, Dr. drg. Widjijono, SU

2013 | Disertasi | S3 Kedokteran Gigi

Fluoride plays an important roles in reducing dental caries by improving remineralization and strengthening email through fluoroapatite forming which is more resistant to acid. The available fluoride dosage form have disadvantages due to its difficulties to control dose and to overcome first hepatic metabolism. Fluoride systemic delivery system to maintain therapeutic plasma concentration need to be developed, and one attractive candidate is transdermal route. Aims this research were to develop patch containing natrium fluoride, to find fluoride concentration on blood and teeth after application and also to study its biocompatibility. Quasy experimental design with post test only control group as research approach was used. Serial transport test with vertical diffusion cell was used as the instrument to examine in vitro skin permeation test with full thickness rat skin as membrane between donor and receptor compartment. Donor was filled with either fluoride solution, fluoride added chemical enhancer and fluoride patch. Sample was taken for time interval of 8, 24 and 48 hours and fluoride concentration was measured by Potensiometer Spesific Fluoride Ion. An in vivo experimentation was done by appliying NaF patch onto dorsal mouse skin and blood sample was taken after 8, 24 and 48 hours to determine fluoride concentration. Teeth was extracted two weeks after application to determine fluoride. Treatment group was patch containing NaF 1.000, 750 and 500 ppm, while patch without NaF was used in control group. Skin sensitisation was evaluated visually for biocompatibility test. Research results showed fluoride can penetrate to the skin. Time of transport influences weight of fluoride and enhancer can increase absorption rate of fluoride. Fluoride weight after transport test of NaF patch for 8-48 hours with patch concentration 0, 500, 750 dan 1.000 mg/L (ppm) was 0.06-0.18 μg; 0.33-0.94 μg; 0.72-1.23 μg; 0.88-1.73 μg. In vivo experiment showed fluoride concentration in blood plasma after application of NaF patch for 0, 8, 24 and 48 hours were 3.9-4.11 ppb; 4.46-9.5 ppb; 4.18-13.4 ppb and 3.9-5.6 ppb respectively. Repeated Anova showed significantly differences fluoride concentration based on patch fluoride concentration and time of application (p<0,05). Fluoride concentration on teeth after two weeks of patch application 0, 750, 500 and 1.000 ppm were 0.53 μg/mg; 0.96 μg/mg; 1.03 μg/mg; 0.77 μg/mg respectively. Sensitivity test showed weak erythema and this condition can still be approved as safe.

Fluoride plays an important roles in reducing dental caries by improving remineralization and strengthening email through fluoroapatite forming which is more resistant to acid. The available fluoride dosage form have disadvantages due to its difficulties to control dose and to overcome first hepatic metabolism. Fluoride systemic delivery system to maintain therapeutic plasma concentration need to be developed, and one attractive candidate is transdermal route. Aims this research were to develop patch containing natrium fluoride, to find fluoride concentration on blood and teeth after application and also to study its biocompatibility. Quasy experimental design with post test only control group as research approach was used. Serial transport test with vertical diffusion cell was used as the instrument to examine in vitro skin permeation test with full thickness rat skin as membrane between donor and receptor compartment. Donor was filled with either fluoride solution, fluoride added chemical enhancer and fluoride patch. Sample was taken for time interval of 8, 24 and 48 hours and fluoride concentration was measured by Potensiometer Spesific Fluoride Ion. An in vivo experimentation was done by appliying NaF patch onto dorsal mouse skin and blood sample was taken after 8, 24 and 48 hours to determine fluoride concentration. Teeth was extracted two weeks after application to determine fluoride. Treatment group was patch containing NaF 1.000, 750 and 500 ppm, while patch without NaF was used in control group. Skin sensitisation was evaluated visually for biocompatibility test. Research results showed fluoride can penetrate to the skin. Time of transport influences weight of fluoride and enhancer can increase absorption rate of fluoride. Fluoride weight after transport test of NaF patch for 8-48 hours with patch concentration 0, 500, 750 dan 1.000 mg/L (ppm) was 0.06-0.18 μg; 0.33-0.94 μg; 0.72-1.23 μg; 0.88-1.73 μg. In vivo experiment showed fluoride concentration in blood plasma after application of NaF patch for 0, 8, 24 and 48 hours were 3.9-4.11 ppb; 4.46-9.5 ppb; 4.18-13.4 ppb and 3.9-5.6 ppb respectively. Repeated Anova showed significantly differences fluoride concentration based on patch fluoride concentration and time of application (p<0,05). Fluoride concentration on teeth after two weeks of patch application 0, 750, 500 and 1.000 ppm were 0.53 μg/mg; 0.96 μg/mg; 1.03 μg/mg; 0.77 μg/mg respectively. Sensitivity test showed weak erythema and this condition can still be approved as safe.

Kata Kunci : Larutan NaF; larutan NaF dengan enhancer; plester NaF; konsentrasi fluorida dalam plasma darah; konsentrasi fluorida dalam gigi; sensitifitas plester