PEMANFAATAN YOGHURT UBI JALAR UNGU (Ipomoea batatas Lamb.) “ YUJU ” UNTUK PERBAIKAN KADAR TRIGLISERIDA DAN KOLESTEROL TOTAL PADA TIKUS Sprague Dawley YANG DIBERI DIET TINGGI LEMAK

QISTHIRA SWASTI AMIRINA, Dr. Ir. Nurliyani, MS.

2013 | Skripsi | GIZI KESEHATAN

ke tahun terus meningkat. PJK diinisiasi oleh adanya peningkatan kadar kolesterol total dan trigliserida yang biasa disebut hiperlipidemia. Pemanfaatan pangan fungsional berbasis lokal sebagai alternatif upaya preventif dan kuratif perlu dikembangkan sebagai terapi suportif di samping terapi farmakologis yang dirasa kurang komprehensif. Yoghurt ubi jalar ungu (YUJU) merupakan salah satu pangan fungsional yang mengandung Bakteri Asam Laktat (BAL), antosianin, dan serat yang berperan dalam perbaikan kolesterol total dan trigliserida. Penelitian seputar yoghurt, antosianin dan serat ubi jalar ungu sudah banyak dilakukan namun dalam bentuk produk yoghurt ubi jalar ungu belum pernah dilakukan. Tujuan: Mengetahui pengaruh pemberian yoghurt ubi jalar ungu “ YUJU” terhadap kadar kolesterol total dan trigliserida tikus Sprague Dawley yang diberi diet tinggi lemak. Metode: Jenis penelitian quasi eksperimental dengan rancangan penelitian pretest dan posttest with control group design.Sebanyak 30 ekor tikus Sprague Dawley jantan umur 2 bulan dibagi menjadi 5 kelompok, yaitu kelompok KON (aquadest), YJ2 (YUJU 2mL), YJ4 (YUJU 4mL), SVT (simvastatin 0,8 mg), YJ4+SVT (YUJU 4mL+simvastatin). Kemudian dilanjutkan dengan pemberian intervensi selama 4 minggu. Analisis kadar kolesterol total dan trigliserida dilakukan pada minggu terakhir pemberian pakan tinggi lemak (pretest), 2 minggu setelah pemberian intervensi (midtest) dan akhir intervensi (posttest). Data dianalisis menggunakan ANOVA dan Kruskal Wallis (non parametric test) untuk mengetahui apakah terdapat perbedaan bermakna antar kelompok perlakuan. Hasil: Tidak terdapat perbedaan yang bermakna pada kadar kolesterol total dan trigliserida antar kelompok perlakuan setelah pemberian intervensi namun terdapat penurunan yang signifikan pada setiap kelompok perlakuan. Kesimpulan: Pemberian yoghurt ubi jalar ungu “YUJU” tidak dapat menurunkan kadar kolesterol total dan trigliserida serum tikus Sprague Dawley yang diberi diet tinggi lemak. Namun terdapat penurunan kadar kolesterol total dan trigliserida dari pretest hingga posttest.

year to year is increasing. CHD initiated by an increase in blood total cholesterol and triglycerides level (hyperlipidemia). Utilization of locally-based functional foods as a preventive and curative alternatives need to be developed as a supportive therapy in addition to pharmacological therapy that is less comprehensive. Purple sweet potato yoghurt is one of the functional food containing lactic acid bacteria, anthocyanin, and dietary fibers that play a role in the improvement of total cholesterol and triglycerides. Research about the yoghurt, anthocyanins and purple sweet potato fiber has been done but in the form of purple sweet potato yoghurt products has not been done yet. Objective: To determine the effect of purple sweet potato yoghurt \"YUJU\" on levels of total cholesterol and triglycerides Sprague Dawley rats fed a high-fat diet. Method: The quasi experimental study with a pretest and posttest study design with control group design.There were 30 Sprague Dawley male rats aged 2 months were divided into 5 groups, there were: KON (aquadest), YJ2 (YUJU 2ml), YJ4 (YUJU 4ml), SVT (simvastatin 0.8 mg), YJ4 + SVT (YUJU 4ml+simvastatin). Then continued with the intervention for 4 weeks. Analysis of total cholesterol and triglycerides made in the last week of high-fat feeding (pretest), 2 weeks after be given by the intervention (midtest) and end of the intervention (posttest). Data were analyzed using ANOVA and Kruskal Wallis (non-parametric test) to determine whether there were significant differences between treatment groups. Results: There were no significant differences in total cholesterol and triglycerides between treatment groups after the intervention, but there is a decreasing trend in each treatment group. Conclusions: Purple sweet potato yoghurt \" YUJU \" can not lower total cholesterol and triglyceride serum Sprague Dawley rats fed a high-fat diet. However, there was a decrease in total cholesterol and triglycerides from pretest to posttest.

Kata Kunci : hiperlipidemia, yoghurt ubi jalar ungu, BAL, antosianin, serat