Pengaruh kerusakan sel Beta pankreas terhadap beda tegangan listrik titik akupuntur :: Suatu kajian biofisik dan morfologis pada tikus putih (Rattus norvegicus)

SAGIRAN, Prof.dr. Soedjono Aswin, PhD

2001 | Tesis | S2 Kedokteran Dasar

Ilmu Akupunktur telah lama dikembangkan sebagai terapi alternatif terhadap gangguan fungsi organ tubuh. Diagnosis terhadap gangguan organ tertentu dapat dilakukan melalui pemeriksaan biofisik titik-titik tertentu pula. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian zat aloksan monohidrat dengan dosis bertingkat terhadap derajat kerusakan sel p pankreas dan perubahan beda tegangan listrik titik akupunktur Subelakang pankreas, serta mengamati hubungan kerusakan pankreas dengan perubahan beda tegangan listrik titik akupunktur. Subjek penelitian ini 12 ekor tikus (Rattus norvegicus) jantan, dibagi menjadi 4 kelompok secara random sederhana, yaitu kelompok I (kontrol) mendapat suntikan aquabidestilata (intraperitoneal); kelompok II (perlakuan 1) mendapat suntikan aloksan 70 mgkg BB (intraperitoneal); kelompok III (perlakuan 2) mendapat suntikan aloksan 140 mgkg BB (intraperitoneal) dan kelompok N (perlakuan 3) mendapat suntikan aloksan 210 mgkg BB (intraperitoneal). Masing-masing kelompok terdiri atas 3 ekor tikus. Tikus dipelihara, dan pada hari ke 7, dilakukan perekaman beda tegangan listrik titik akupunktur Su-belakang pankreas, dengan menggunakan alat akupunkturmeter. Setelah perekaman selesai, tikus dimatikan, kemudian diambil pakreasnya dan dibuat sediaan histologis dengan pemulasan Victoria blue, yang dapat mewarnai granula insulin pada sel p. Data yang diperoleh dianalisis dengan ANAVA satujalan, Uji-t, dan regresi linier. Hasil-hasil penelitian menunjukkan bahwa, pemberian aloksan monohidrat menyebabkan kerusakan sel p pankreas, sehingga rasio sel p per jumlah sel di satu pulau Langerhans pada kelompok perlakuan (0,3 10 5 0,073) lebih kecil (p

Acupuncture has been developed for a long time ago as an alternative therapy to cure many disturbances of the body. Diagnostic effort of organ disorder can also be done through the biophysical study of the acupuncture point.The aim of this study is to reveal the effect of alloxan monohydrate treated gradually on pancreatic damage and electrical voltage changes of acupuncture point (Pi Shu), to observe the effect of organ damage on electrical voltage changes of the related-acupoint. The subjects, 12 male rats, were randomly divided into four groups, i.e. group I (control) was treated by aquabidestilata; group IT (treatment 1) was treated by alloxan 70 mgkg BW; group 111 (treatment 2) was treated by alloxan 140 m a g BW and group IV (treatment 3) was treated by alloxan 210 mgkg BW. All these treatments were done intrapentoneally. Each group consisted of 3 rats. By the 7& day of treatment, electrical voltage changes were observed. Recording was taken through the acupuncture points of the pancreas in the back (Pi Shu) by Acupuncturemeter. After the recording were accomplished, the rats were killed, followed by taking out their pancreas and then we= processed by Victoria blue staining for microscopic features preparations of p cells in islets of Lmgerhans. The data collected then were analysed with one way ANOVA, t-Test and Linear Regression. The results of this study showed that gradually treatment of alloxan monohydrate caused pancreas J3 cell damages, so the ratio of pcell per cell number in one islet of treatment group is (0,3 10 5 0,073) less significantly (p<0,05) than the ratio of control group (0,571 5 0,133). The electrical voltage changes in treatment group is (33,084 5 7,344) higher significantly (p<0,05) than the changes in control group (13,653 5 5,628). The effect of pancreatic damage on the skin electrical voltage changes of acupuncture point has been statistically analyzed by Lineur Regression, showed a significant difference in group 111 (group which were treated by alloxan 140 mgkg BW). These results indicate that gradual treatment of alloxan monohydrate treated gradually cause pancreatic damage and electrical voltage changes of acupuncture point (pi Shu). There is a significan difference in f3 cell ratio per number of entire islet cells, one group differs from another. So does in electri'cal voltage changes. It is suggested that there is a relationship between organ damage and electrical voltage changes of the related-acupoints.

Kata Kunci : Akupuntur,Kerusakan Pankreas, alloxan, pancreatic p cell, electrical voltage, acupuncture point.