IDENTIFIKASI ENTEROBACTERIACEAE PADA SUSU KAMBING PERANAKAN ETTAWAH (PE) DI PETERNAKAN SAHABAT ALAM SEJATI, KALASAN, SLEMAN

DIAN MEIDIDEWI NURAINI, Dr. drh.Agnesia Endang Tri Hastuti Wahyuni, M.Si.

2013 | Skripsi | KEDOKTERAN HEWAN

Susu kambing peranakan ettawah (PE) memiliki kandungan yang bermanfaat bagi tubuh manusia dan konsumen terus meningkat. Hal ini menyebabkan peternakan kambing PE memiliki prospek yang baik. Perkembangan peternakan kambing PE di Yogyakarta cukup tinggi, salah satunya Peternakan “Sahabat Alam Sejati” di Kalasan, Sleman. Kendala utama yang menghambat perkembangan peternakan kambing PE adalah mastitis. Salah satu penyebab mastitis adalah bakteri dari famili Enterobactaeriaceae. Penelitian ini bertujuan untuk mengidentifikasi bakteri Enterobacteriaceae yang dapat ditemukan pada susu kambing PE di peternakan “Sahabat Alam Sejati”. Sebanyak 40 sampel dari 20 ekor kambing PE digunakan dalam penelitian. Sampel diuji status mastitisnya menggunakan reagen IPB-1. Isolasi Enterobacteriaceae melalui media Mac Conkey agar (MCA). Koloni yang tumbuh, baik koloni dengan warna merah maupun transparan di MCA di warnai menggunakan pengecatan Gram untuk melihat morfologi sel. Koloni dari MCA kemudian diiolasi ke media eosin metylen blue (EMB), triple sugar iron (TSI) dan diuji indol, methyl red, voges proskauer, citrat (IMViC), glukosa, sukrosa, laktosa, dan urea. Hasil uji mastitis menunjukan 37 sampel (92,5%) mastitis subklinis, 2 sampel (5%) mastitis klinis dan 1 sampel (2,5%) normal. Pengecatan bakteri menunjukan 7 morfologi sel berbentuk basil. Hasil identifikasi lanjut MCA, EMB, TSI, IMViC, gula-gula dan urea menunjukan 2 isolat adalah Klebsiella oxytoca, 2 isolat Klebsiella pneumonia, 1 isolat Seratia marcescens, dan 1 isolat Escherichia coli pada susu mastitis subklinis serta ditemukan 1 isolat Citrobacter freundii pada susu normal.

number of consumer keep increasing lately. Goat farm prospect is still growing in Indonesia following the number of consumer. The development of goat farm in Yogyakarta is high enough. One of the success farm in Yogyakarta is Peternakan “Sahabat Alam Sejati” in Kalasan, Sleman. The principal problem in this farm is mastitis. Most of mastitis on goat is caused by infection of bacteria including Enterobacteriaceae family. This study aims to identify Enterobacteriaceae in goat’s milk from Peternakan “Sahabat Alam Sejati”. This study used 40 samples from 20 goats. Samples were tested by IPB-1 reagent to determine the mastitis status and inoculated on Mac Conkey agar (MCA). The colonies from MCA were stained by Gram stain to determine cell morphology. The colonies from MCA were isolated on eosin methylen blue (EMB), triple sugar iron (TSI) and were tested by indol, methyl red, voges proskauer, citrat (IMViC), glucosa, sucrose, lactose broth, and urea agar. The result of mastitis test showed 37 samples (92,5%) were subclinical mastitis, 2 samples (5%) were clinical mastitis and 1 sample (2,5%) were normal. Staining showed 7 colonies were basil. The identification result on MCA, EMB, TSI, IMViC, sugars broth and urea agar showed that 2 colonies were Klebsiella pneumoniae, 2 colonies were Klebsiella oxytoca, and 1 colony was Escherichia coli found on the subclinical mastitis milk. Besides that, 1 colony was identified as Citrobacter freundii found on normal milk.

Kata Kunci : Susu kambing PE, Enterobacteriaceae