STUDI TENTANG GAMBARAN HISTOLOGIS LIMPA DAN PARU-PARU MENCIT TOKSOPLASMOSIS YANG DITERAPI DENGAN INTERTRIM® DAN TOXOPLASMANUUR

DYAH NOVIANDARI, Prof. Dr. drh. Sri Hartati, S.U.

2013 | Skripsi | KEDOKTERAN HEWAN

Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian Intertrim® dan Toxoplasmanuur pada mencit toksoplasmosis terhadap limpa dan paru-paru secara histologis. Penelitian ini telah dilakukan di Laboratorium Parasitologi dan Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta. Mencit sebanyak 22 ekor secara acak dibagi menjadi 4 kelompok. Kelompok 1 sebagai kontrol tanpa infeksi sebanyak 4 ekor, kelompok II sebagai kontrol yang diinfeksi secara intraperitoneal dengan 103 takizoit/ekor sebanyak 4 ekor, kelompok III sebanyak tujuh ekor diinfeksi dengan 103 takizoit/ekor secara intraperitoneal dan diberikan Toxoplasmanuur 8 mg/ekor/hari mencit peroral, kelompok IV sebanyak tujuh ekor diinfeksi dengan 103 takizoit/ekor secara intraperitoneal dan diberikan obat Intertrim® 0,001ml/kgBB/hari peroral. Hasil penelitian menunjukkan mencit kelompok I bertahan sampai penelitian selesai, kelompok II mati diantara hari kelima sampai ketujuh, kelompok III mati diantara hari keempat sampai ketujuh, dan kelompok IV bertahan sampai penelitian selesai. Mencit yang mati kemudian dinekropsi, diambil organ limpa dan paruparu kemudian dimasukkan kedalam formalin 10%, dan dibuat preparat histologis. Data yang diperoleh dianalisis secara deskriptif. Hasil dari penelitian ini dapat disimpulkan bahwa infeksi takizoit 103 pada organ paru-paru dan limpa dapat menyebabkan nekrosis, hemoragi, edema dan pneumonia, pemberian Toxoplasmanuur tidak mampu mengobati infeksi T. gondii, dan pemberian Intertrim® mampu mengobati dan memperpanjang daya hidup mencit toksoplasmosis.

The aim of this study was to identify the influence of the Intertrim and Toxoplasmanuur histologically toward the spleen and the lungs of the mice with Toxoplasmosis. This study was conducted in Laboratory of Parasitologi and Laboratory of Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta. Twenty two mice were used in this study. They were randomly divided into 4 groups. Group I which consisted of 4 mice, played as a control group. This group received no Toxoplasma gondii infections. Group II that played as another control group, was infected with 103 takizoit/mice intraperitoneally. Group III that consisted of 7 mices, was infected with 103 takizoit/mice intraperitoneally and was given Toxoplasmanuur 8mg/mice/day orally. Group IV that consisted of 7 mice, was infected with 103 takizoit/mice intraperitoneally and was given Intertrim® 0,001ml/kg of body weight/day orally. The result of this study showen that the first group could survived until the end of this study, the second group died between the 5th-7th day of this study, the third group died between the 4th-7th day of this study, and the fourth group could survived until the end of this study. The died mice were necropted, spleen and lungs were collected, put inside the tubes with formalin 10%, and made into histological preparat. The data was analyzed descriptively. Based on the result of this study, we can conclude that infection caused by 103 takizoit/mice was leading to necrosis, haemorraghe, oedema, and pneumonia. Toxoplasmanuur that given couldn’t treat the infection whereas the Intertrim® that given could effectively treat the infection and prolonged the life of mice with toxoplasmosis.

Kata Kunci : Toksoplasmosis, Toxoplasmanuur, Intertrim®, histologi